表皮生长因子增强N-乙基-N-亚硝基脲诱导的叙利亚仓鼠胚胎细胞的形态转化。
Epidermal growth factor enhances N-ethyl-N-nitrosourea-induced morphological transformation of Syrian hamster embryo cells.
作者信息
de Kok A J, Sips H M, Den Engelse L, Simons J W
机构信息
Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
出版信息
Carcinogenesis. 1988 Apr;9(4):661-4. doi: 10.1093/carcin/9.4.661.
To improve the usefulness of the Syrian hamster embryo (SHE) cell transformation system as a short-term test, it was investigated whether the variation in results due to serum variability could be reduced by the addition of epidermal growth factor (EGF). It was found that EGF-significantly (3-7-fold) enhanced the frequency of morphological transformation induced by N-ethyl-N-nitrosourea or benzo[a]pyrene if added to growth medium supplemented with a batch of serum which had a low ability to support transformation. Furthermore, addition of EGF to the assay medium enabled the demonstration of dose dependence of transformation with relatively small group sizes (up to 2000 colonies). Finally, it was observed that the transformed phenotype was easier to recognize in the presence of EGF. These data suggest that routine addition of EGF to the assay medium might reduce variability and enhance sensitivity of the SHE transformation assay.
为提高叙利亚仓鼠胚胎（SHE）细胞转化系统作为短期检测方法的实用性，研究了添加表皮生长因子（EGF）是否能减少因血清变异性导致的结果差异。结果发现，如果将EGF添加到补充了一批支持转化能力较低的血清的生长培养基中，它能显著（3至7倍）提高由N-乙基-N-亚硝基脲或苯并[a]芘诱导的形态转化频率。此外，在检测培养基中添加EGF能够在相对较小的样本量（最多2000个集落）下证明转化的剂量依赖性。最后，观察到在存在EGF的情况下，转化表型更容易识别。这些数据表明，在检测培养基中常规添加EGF可能会减少变异性并提高SHE转化检测的灵敏度。
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