Induces Gene Expression in Human Acute Leukemia Jurkat T-Cells.

OBJECTIVE

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease resulting from the accumulation of genetic changes that affect the development of T-cells. The precise role of lymphoid enhancer-binding factor 1 () in T-ALL has been controversial since both overexpression and inactivating mutations have been reported to date. Here, we investigate the potential gene targets of in the Jurkat human T-cell leukemia cell line.

MATERIALS AND METHODS

We used small interfering RNA (siRNA) technology to knock down in Jurkat cells and then compared the gene expression levels in the knockdown cells with non-targeting siRNA-transfected and non-transfected cells by employing microarray analysis.

RESULTS

We identified , a tumor suppressor gene, as the most significantly downregulated gene in knockdown cells, and we further confirmed its downregulation by real-time quantitative polymerase chain reaction (qRT-PCR) in mRNA and at protein level by western blotting.

CONCLUSION

Our results revealed that is positively regulated by in Jurkat cells, which indicates the capability of as a tumor suppressor and, together with previous reports, suggests that exhibits a regulatory role in T-ALL via not only its oncogenic targets but also tumor suppressor genes.