Analytical Chemistry-Biointerphases, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
Biointerphases. 2020 Jun 25;15(3):031014. doi: 10.1116/6.0000240.
Testing the adhesion of marine biofilm formers on bioresistant coatings is important to determine their fouling-release and antifouling properties. A dynamic attachment assay for the marine bacterium Cobetia marina (C. marina) was developed to test the adhesion on coatings and bioresistant surfaces. With well-defined culture conditions, the reproducibility of the microfluidic accumulation experiments with C. marina was verified using self-assembling monolayers as model surfaces. The assay discriminated the attachment of C. marina on four different surfaces with different wettability and protein resistances. In addition to these benchmark experiments on self-assembled monolayers, the adhesion of C. marina on polyglycerol coatings with different thicknesses was investigated.
测试海洋生物膜形成物在生物抗性涂层上的附着情况对于确定其防污释放和防污性能非常重要。为了测试海洋细菌科尔比氏菌（C. marina）在涂层和生物抗性表面上的附着情况，开发了一种用于科尔比氏菌的动态附着测定法。通过使用自组装单分子层作为模型表面，在明确的培养条件下，验证了使用微流控累积实验对科尔比氏菌进行重复性测试的可行性。该测定法区分了科尔比氏菌在具有不同润湿性和蛋白质抗性的四种不同表面上的附着情况。除了在自组装单分子层上进行这些基准实验外，还研究了科尔比氏菌在不同厚度的聚甘油涂层上的附着情况。
