Segerson E C
Department of Animal Science, North Carolina A & T State University, Greensboro 27411.
Biol Reprod. 1988 Mar;38(2):256-63. doi: 10.1095/biolreprod38.2.256.
Ovine uterine luminal protein (ULP) obtained from ewes on Day 14 of pregnancy suppressed blastogenesis of interleukin-2 (IL-2)-dependent T-lymphocytes. Varying concentrations of ULP (4 to 96 micrograms/ml) followed by a 1:4 dilution of human IL-2 suppressed (p less than 0.001) IL-2 blastogenesis of IL-2-dependent T-lymphocytes with mean percentage of control values ranging from 55.3 to 34.5% (44.7 to 65.5% suppression, respectively). For two experiments, IL-2 was added at varying times (zero to 4 h) after the addition of ULP to cultures. Suppression was independent of IL-2 addition time. Mean (+/- SEM) percentage of control values for combined time periods for 40 and 120 micrograms ULP/ml were 43.3 +/- 1.0 and 27.8 +/- 1.9%, respectively. In another experiment, additional IL-2 (1:2 vs. 1:4 dilution) reduced (p less than 0.01) the immunosuppressive effect of ULP. Sephacryl S-200 chromatography of ULP and the phytohemagglutinin (PHA) blastogenesis assay revealed significant immunosuppressive activity for Fractions I (greater than or equal to 248,000 Mr), III (70,000 Mr), and V (14,000 Mr). These fractions also suppressed (p less than 0.001) IL-2-mediated blastogenesis of T-lymphocytes. Results indicate that immunosuppression of PHA-treated lymphocytes was associated with an alteration of the IL-2 system.
从怀孕第14天的母羊中获得的绵羊子宫腔蛋白（ULP）可抑制白细胞介素-2（IL-2）依赖性T淋巴细胞的增殖。不同浓度的ULP（4至96微克/毫升），随后将人IL-2按1:4稀释，可抑制（p<0.001）IL-2依赖性T淋巴细胞的IL-2增殖，其平均对照值百分比范围为55.3%至34.5%（分别抑制44.7%至65.5%）。在两项实验中，在向培养物中添加ULP后的不同时间（0至4小时）添加IL-2。抑制作用与IL-2添加时间无关。对于40和120微克ULP/毫升的合并时间段，对照值的平均（±SEM）百分比分别为43.3±1.0%和27.8±1.9%。在另一项实验中，额外的IL-2（1:2与1:4稀释）降低了（p<0.01）ULP的免疫抑制作用。对ULP进行Sephacryl S-200层析以及植物血凝素（PHA）增殖试验显示，组分I（≥248,000 Mr）、III（70,000 Mr）和V（14,000 Mr）具有显著的免疫抑制活性。这些组分也抑制（p<0.001）了IL-2介导的T淋巴细胞增殖。结果表明，PHA处理的淋巴细胞的免疫抑制与IL-2系统的改变有关。
