Hwang Han-Sung, Maeng Yong-Sun
Department of Obstetrics and Gynecology, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Republic of Korea.
Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea.
Stem Cells Int. 2020 Jun 8;2020:8403192. doi: 10.1155/2020/8403192. eCollection 2020.
Preeclampsia is a syndrome characterized by deterioration of either the maternal condition or the fetal condition. The adverse intrauterine environment made by preeclampsia results into intrauterine growth restriction and increased risk of a variety of diseases in future life. Given the adverse environment of fetal circulation made in the preeclamptic condition, and the role of mesenchymal stem cell (MSC) as a multipotent progenitor cell, we hypothesized that MSCs derived from human umbilical cord blood (hUCB-MSCs) obtained from preeclampsia are adversely altered or affected compared with normal pregnancy. The aim of this study was to analyze the biological characteristics and compare the functional abilities and gene expression patterns of hUCB-MSCs originating from pregnant women with and without severe preeclampsia. hUCB-MSCs were isolated and cultured from 28 pregnant women with severe preeclampsia and 30 normal pregnant women. hUCB-MSCs obtained from women with preeclampsia were less proliferative and more senescent and had lower telomerase activity and higher ROS activity than cells from women with normal pregnancy. In addition, many senescence-related differentially expressed genes (DEGs) were identified by analysis of microarray gene expression profiles and significantly associated with the Gene Ontology term cell aging. In conclusion, hUCB-MSCs obtained from women with preeclampsia showed the poorly proliferative, more senescent, and decreased telomerase activity, and these characters may be related with functional impairment of MSC from preeclampsia compared with cells from normal pregnancy.
子痫前期是一种以母体状况或胎儿状况恶化特征的综合征。子痫前期所造成的不良宫内环境会导致胎儿宫内生长受限，并增加未来患各种疾病的风险。鉴于子痫前期状况下胎儿循环的不良环境，以及间充质干细胞（MSC）作为多能祖细胞的作用，我们推测，与正常妊娠相比，子痫前期患者的人脐带血间充质干细胞（hUCB-MSCs）会发生不良改变或受到影响。本研究的目的是分析重度子痫前期孕妇与正常孕妇来源的hUCB-MSCs的生物学特性，并比较其功能能力和基因表达模式。从28例重度子痫前期孕妇和30例正常孕妇中分离培养hUCB-MSCs。与正常妊娠孕妇的细胞相比，子痫前期孕妇的hUCB-MSCs增殖能力更弱、衰老程度更高，端粒酶活性更低，活性氧（ROS）活性更高。此外，通过微阵列基因表达谱分析鉴定出许多与衰老相关的差异表达基因（DEGs），且这些基因与基因本体论术语“细胞衰老”显著相关。总之，子痫前期孕妇的hUCB-MSCs表现出增殖能力差、衰老程度高和端粒酶活性降低的特点，与正常妊娠孕妇的细胞相比，这些特征可能与子痫前期患者的间充质干细胞功能受损有关。
