Ziętek Jerzy, Dzięgiel Beata, Wójcik Alicja, Wilczyńska Anna, Adaszek Łukasz, Winiarczyk Stanisław
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin Poland.
J Vet Res. 2020 May 27;64(2):313-318. doi: 10.2478/jvetres-2020-0038. eCollection 2020 Jun.
The prohibition of antibiotic use in edible snails obligates breeders to treat bacterial infections by different means, of which a common one is a bath in Gram-positive- and partially Gram-negative-bactericidal ethacridine lactate solution. The aim of the study was to determine the effect of bathing Müller snails in a 0.1% aqueous solution of ethacridine lactate on selected physiological parameters of haemolymph.
The study included 80 snails, divided into two equal groups (study and control). The study group was subjected to bathing in ethacridine lactate and the control group to bathing in tap water. Both groups were treated daily for seven days. The number of haemocytes in the haemolymph, the activity of alanine (ALT) and aspartate (AST) aminotransferases, and the concentration of urea were determined.
In the study group, after exposure to ethacridine lactate solution an increase in ALT activity, changes in the De Ritis ratio, an increase in the amount of haemocytes, and a decrease in body weight were found. No such changes were detected in the control group snails or in animals after the first bath.
Multiple applications of a 0.1% ethacridine lactate bath may adversely affect Müller snails.
食用蜗牛中抗生素使用的禁令使养殖者必须通过不同方法治疗细菌感染，其中一种常见方法是将蜗牛浸泡在革兰氏阳性菌和部分革兰氏阴性菌杀菌的乳酸依沙吖啶溶液中。本研究的目的是确定将米勒蜗牛浸泡在0.1%乳酸依沙吖啶水溶液中对血淋巴选定生理参数的影响。
该研究包括80只蜗牛，分为两个相等的组（研究组和对照组）。研究组蜗牛浸泡在乳酸依沙吖啶溶液中，对照组蜗牛浸泡在自来水中。两组均每天处理7天。测定血淋巴中的血细胞数量、丙氨酸（ALT）和天冬氨酸（AST）转氨酶活性以及尿素浓度。
在研究组中，暴露于乳酸依沙吖啶溶液后，发现ALT活性增加、德瑞蒂斯比值变化、血细胞数量增加以及体重下降。对照组蜗牛或首次浸泡后的动物未检测到此类变化。
多次应用0.1%乳酸依沙吖啶浸泡可能会对米勒蜗牛产生不利影响。
