College of Resources and Environment, Southwest University, Chongqing, China.
J Appl Microbiol. 2020 Dec;129(6):1684-1692. doi: 10.1111/jam.14752. Epub 2020 Jul 11.
Biological techniques can manage plant diseases safely and in environmentally friendly ways, but their efficacy needs improvement. It is of the utmost importance to search for powerful microbes for the effective control of plant diseases.
Unheated self-digestive solutions (SDS) that were heated at 100°C for 30 min(H-SDS) or stored for 12 months at room temperature (S-SDS) were prepared from Lysobacter enzymogenes LE16 broth culture to study their potential as biocontrol agents. This bacterium produced protease, phosphatase, lysozyme and siderophores in pure culture as well as 12 secondary metabolites including novel antibiotics lysobactin, WAP-8294A and mupirocin determined based on the antiSMASH 5.0.0 blast database. A poison plate assay revealed the antagonistic activities of SDS, H-SDS and S-SDS against an animal pathogenic bacterium Staphylococcus aureus, a phytopathogenic bacterium Pseudomonas syringae pv. tabaci, and numerous plant pathogenic fungi and oomycetes, including Colletotrichum gloeosporioides, Penicillium italicum, Alternaria alternate, Rhizoctonia solani, Didymella bryoniae, Sclerotinia sclerotiorum, Phytophthora nicotianae and Phytophthora capsici. The greenhouse experiment showed that SDS was highly effective in controlling pepper blight disease, which is caused by P. capsici. Compared with only pathogen inoculation, the application of SDS to the soil in preventive or curative treatments significantly reduced the disease incidence and index with relatively high control efficacy of 86·2-93·1%.
SDS enriched lytic enzymes, siderophores and antibiotics, has a wide antimicrobial spectrum, and shows potential as a new, safe and effective biocontrol agent against plant diseases.
Autolysates of the new biocontrol bacterium L. enzymogenes LE16 demonstrated the potential for industrial production and commercial use as a promising biocontrol agent in agriculture.
生物防治技术能够安全、环保地防治植物病害，但需要提高其效果。寻找有效的控制植物病害的强大微生物至关重要。
从溶杆菌 LE16 发酵液中制备未经加热的自消化溶液（SDS）和经 100°C 加热 30 分钟（H-SDS）或在室温下储存 12 个月（S-SDS），以研究其作为生物防治剂的潜力。该细菌在纯培养中产生蛋白酶、磷酸酶、溶菌酶和铁载体，以及根据 antiSMASH 5.0.0 爆炸数据库确定的 12 种次级代谢产物，包括新型抗生素 Lysobactin、WAP-8294A 和 Mupirocin。毒板试验显示 SDS、H-SDS 和 S-SDS 对动物病原菌金黄色葡萄球菌、植物病原菌丁香假单胞菌 pv. 番茄和许多植物病原菌真菌和卵菌，包括炭疽菌、意大利青霉、交链孢菌、立枯丝核菌、菟丝子菌、核盘菌、烟草疫霉和辣椒疫霉具有拮抗活性。温室试验表明，SDS 对由 P. capsici 引起的辣椒疫病具有高效防治作用。与仅接种病原菌相比，SDS 预防性或治疗性施用于土壤可显著降低病害发生率和指数，相对控制效果为 86.2-93.1%。
SDS 富含溶菌酶、铁载体和抗生素，具有广谱抗菌谱，有望成为防治植物病害的新型、安全、有效生物防治剂。
新的生物防治细菌溶杆菌 LE16 的自溶产物具有工业化生产和商业应用的潜力，有望成为农业中一种有前途的生物防治剂。
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