乳腺非典型增生癌前病变的分子指纹图谱

Molecular fingerprint of precancerous lesions in breast atypical hyperplasia.

作者信息

Zheng Chao, Jia Hong Ying, Liu Li Yuan, Wang Qi, Jiang Hong Chuan, Teng Li Song, Geng Cui Zhi, Jin Feng, Tang Li Li, Zhang Jian Guo, Wang Xiang, Wang Shu, Alejandro Fernandez-Escobar, Wang Fei, Yu Li Xiang, Zhou Fei, Xiang Yu Juan, Huang Shu Ya, Fu Qin Ye, Zhang Qiang, Gao De Zong, Ma Zhong Bing, Li Liang, Fan Zhi Min, Yu Zhi Gang

机构信息

Department of Breast Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Center of Evidence-based Medicine, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

出版信息

J Int Med Res. 2020 Jun;48(6):300060520931616. doi: 10.1177/0300060520931616.

DOI:10.1177/0300060520931616

PMID:32589079

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7325464/

Abstract

OBJECTIVE

To identify atypical hyperplasia (AH) of the breast by shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS), and to explore the molecular fingerprinting characteristics of breast AH.

METHODS

Breast hyperplasia was studied in 11 hospitals across China from January 2015 to December 2016. All patients completed questionnaires on women's health. The differences between patients with and without breast AH were compared. AH breast lesions were detected by Raman spectroscopy followed by the SHINERS technique.

RESULTS

There were no significant differences in clinical features and risk-related factors between patients with breast AH (n = 37) and the control group (n = 2576). Fifteen cases of breast AH lesions were detected by Raman spectroscopy. The main different Raman peaks in patients with AH appeared at 880, 1001, 1086, 1156, 1260, and 1610 cm, attributed to the different vibrational modes of nucleic acids, β-carotene, and proteins. Shell-isolated nanoparticles had different enhancement effects on the nucleic acid, protein, and lipid components in AH.

CONCLUSION

Raman spectroscopy can detect characteristic molecular changes in breast AH lesions, and may thus be useful for the non-invasive early diagnosis and for investigating the mechanism of tumorigenesis in patients with breast AH.

摘要

目的

采用壳层隔离纳米粒子增强拉曼光谱法（SHINERS）鉴别乳腺不典型增生（AH），并探索乳腺AH的分子指纹特征。

方法

2015年1月至2016年12月，在中国11家医院对乳腺增生进行研究。所有患者均完成了关于女性健康的问卷调查。比较有和没有乳腺AH患者之间的差异。通过拉曼光谱法检测AH乳腺病变，随后采用SHINERS技术。

结果

乳腺AH患者（n = 37）与对照组（n = 2576）在临床特征和风险相关因素方面无显著差异。通过拉曼光谱法检测到15例乳腺AH病变。AH患者主要不同的拉曼峰出现在880、1001、1086、1156、1260和1610 cm处，归因于核酸、β-胡萝卜素和蛋白质的不同振动模式。壳层隔离纳米粒子对AH中的核酸、蛋白质和脂质成分具有不同的增强作用。