College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China; Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Chemosphere. 2020 Nov;259:127443. doi: 10.1016/j.chemosphere.2020.127443. Epub 2020 Jun 20.
Rice bran, a super food or health food supplement, contains high arsenic (As) levels. However, the evaluation of relative bioavailability (RBA) or bioaccessibility (BA) is limited in the rice bran. In this study, the As-RBA in rice bran was determined based on mice model and compared to As-BA using in vitro methods. The As-BA from rice bran-amended feed in the gastric, small intestinal, and colon phases were 33.1-56.4%, 50.5-75.6%, and 35.5-71.4%, respectively. The As-BA was adversely associated with bioaccessible Ca and Fe concentrations in the gastrointestinal phases. Similarly, the As-RBA was significant negative relative with Ca, Fe, and Zn concentrations. The As-RBA values were 37.9-65.5%, 41.5-75.6% and 38.7-71.5% based on liver, kidneys, and combined endpoint (liver plus kidneys), respectively. The in vitro-in vivo correlations (IVIVCs) in the gastric (R = 0.392) and colon (R = 0.362) phases were weak. While the IVIVC (R = 0.544) in the small intestinal phase was stronger than those of the gastric and colon phases. In addition, there was no significant difference in As speciation between colonic residual solids and faeces (p > 0.05). This work provides a better view of human health risk evaluation on rice bran As consumption in humans.
米糠是一种超级食物或保健品，含有高浓度的砷 (As)。然而，米糠中相对生物利用度 (RBA) 或生物可及性 (BA) 的评估是有限的。在这项研究中，基于小鼠模型确定了米糠中的 As-RBA，并与体外方法中的 As-BA 进行了比较。米糠饲料在胃、小肠和结肠阶段的 As-BA 分别为 33.1-56.4%、50.5-75.6%和 35.5-71.4%。As-BA 与胃肠道阶段中生物可及的 Ca 和 Fe 浓度呈负相关。同样，As-RBA 与 Ca、Fe 和 Zn 浓度呈显著负相关。基于肝脏、肾脏和联合终点（肝脏加肾脏），As-RBA 值分别为 37.9-65.5%、41.5-75.6%和 38.7-71.5%。胃 (R = 0.392) 和结肠 (R = 0.362) 阶段的体外-体内相关性 (IVIVC) 较弱。而小肠阶段的 IVIVC (R = 0.544) 强于胃和结肠阶段。此外，结肠残留固体和粪便之间的 As 形态没有显著差异 (p > 0.05)。这项工作为人类食用米糠 As 提供了更好的视角，以评估人类健康风险。
