Xiang Jun-Yan, Chi Yan-Yu, Han Jin-Xin, Shi Xinyu, Cai Yong, Xiang Hongyu, Xie Qiuhong
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, China.
School of Life Sciences, Jilin University, Changchun, China.
Front Nutr. 2022 Feb 18;9:822429. doi: 10.3389/fnut.2022.822429. eCollection 2022.
Alcoholic hepatitis (AH) has a high short-term mortality rate. has the potential to ameliorate liver damage and be a source of prebiotics. We aimed to investigate whether extract (SCE) can improve AH and the role of the small intestinal and cecal microbiota and their metabolites. UHPLC-QE-MS was used to analyze the chemical components of SCE. The chronic-plus-binge ethanol feeding model was used to induce AH in mice. H NMR was used to analyze intestinal metabolites. 16S rRNA-based high throughput sequencing was used to evaluate the effects of SCE on intestinal microbiota (IM). Intestinal microbiota transplantation was used to explore the role of IM in SCE treatment of AH. SCE ameliorated AH non-dose-dependently. SCE effectively improved liver inflammation and oxidative/nitrosative stress, strengthened intestinal barrier function, and regulated the composition of IM and the content of short-chain fatty acids (SCFAs) in AH mice. Samples from and SCE-altered IM improved liver status and regulated the IM. The administration of and ameliorated AH to some extent. The administration of and had partial beneficial effects on AH. Collectively, IM and metabolites were closely associated with the improvement of SCE on AH. The possible microbe targets were the growth inhibition of and the expansion of SCFA producers, such as and . can be considered as a safe and effective dietary supplement for the prevention and improvement of AH.
酒精性肝炎（AH）具有较高的短期死亡率。具有改善肝损伤的潜力且是益生元的一个来源。我们旨在研究五味子提取物（SCE）是否能改善AH以及小肠和盲肠微生物群及其代谢产物的作用。采用超高效液相色谱-四极杆飞行时间质谱联用仪（UHPLC-QE-MS）分析SCE的化学成分。采用慢性加暴饮乙醇喂养模型诱导小鼠发生AH。利用核磁共振氢谱（1H NMR）分析肠道代谢产物。基于16S rRNA的高通量测序用于评估SCE对肠道微生物群（IM）的影响。采用肠道微生物群移植来探究IM在SCE治疗AH中的作用。SCE对AH的改善不具有剂量依赖性。SCE有效改善了肝脏炎症和氧化/亚硝化应激，增强了肠道屏障功能，并调节了AH小鼠的IM组成和短链脂肪酸（SCFA）含量。来自……和SCE改变的IM的样本改善了肝脏状态并调节了IM。……和……的给药在一定程度上改善了AH。……和……的给药对AH有部分有益作用。总体而言，IM及其代谢产物与SCE对AH的改善密切相关。可能的微生物靶点是抑制……的生长以及扩大SCFA产生菌，如……和……。SCE可被视为预防和改善AH的一种安全有效的膳食补充剂。
