Binder B, Hattey E, Koch M, Luhan W, Nemeth E, Wick G
Wien Klin Wochenschr. 1977 May 13;89(10):336-41.
A method is described for the purification of horse anti-human lymphocyte serum, whereby absorption of haemagglutinating antibodies was performed with human RBC ghosts. These ghosts were repeatedly used after desorption. Desorption was carried out in glycine-HCl buffer solution pH = 3,0. The advantages of this method consist of a reduction in the amount of RBC needed for absorption and the relatively low loss of lymphocytotoxic antibodies. The final product is effective in an in vitro test system and complies with the requirements for human use.
描述了一种纯化马抗人淋巴细胞血清的方法，该方法通过用人红细胞血影吸收血凝抗体。这些血影在解吸后可重复使用。解吸在pH = 3.0的甘氨酸 - 盐酸缓冲溶液中进行。该方法的优点包括吸收所需红细胞量的减少以及淋巴细胞毒性抗体相对较低的损失。最终产品在体外测试系统中有效且符合人体使用要求。
