College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, China.
Institute of Wild Economic Animals and Plants and State Key Laboratory for Molecular Biology of Special Economical Animals, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
Hereditas. 2020 Jun 26;157(1):24. doi: 10.1186/s41065-020-00137-x.
Sika deer is one of the most popular and valued animals in China. However, few studies have been conducted on the microsatellite of Sika deer, which has hampered the progress of genetic selection breeding. To develop and characterize a set of microsatellites for Sika deer which provide helpful information for protection of Sika deer natural resources and effectively increase the yield and quantity of velvet antler.
We conducted a transcriptome survey of Sika deer using next-generation sequencing technology. One hundred eighty-two thousand two hundred ninety-five microsatellite markers were identified in the transcriptome, 170 of 200 loci were successfully amplified across panels of 140 individuals from Shuangyang Sika deer population. And 29 loci were found to be obvious polymorphic. Number of alleles is from 3 to 14. The expected heterozygosity ranged from 0.3087 to 0.7644. The observed heterozygosity ranged from 0 to 0.7698. The polymorphism information content values of those microsatellites varied ranged from 0.2602 to 0.7507. The marker-trait association was tested for 6 important and kernel characteristics of two-branched velvet antler in Shuangyang Sika deer through one-way analysis of variance. The results showed that marker-trait associations were identified with 8 different markers, especially M009 and M027.
This study not only provided a large scale of microsatellites which were valuable for future genetic mapping and trait association in Sika deer, but also offers available information for molecular breeding in Sika deer.
梅花鹿是中国最受欢迎和最有价值的动物之一。然而，对梅花鹿的微卫星研究甚少，这阻碍了遗传选择育种的进展。为了开发和鉴定一套梅花鹿微卫星标记，为梅花鹿的自然保护提供有价值的信息，并有效地提高鹿茸的产量和质量。
我们使用下一代测序技术对梅花鹿进行了转录组调查。在转录组中鉴定出 182295 个微卫星标记，在来自双阳梅花鹿种群的 140 个个体的面板中成功扩增了 200 个位点中的 170 个。发现 29 个位点明显多态。等位基因数为 3 至 14。期望杂合度范围为 0.3087 至 0.7644。观察杂合度范围为 0 至 0.7698。这些微卫星的多态信息含量值从 0.2602 到 0.7507 不等。通过单向方差分析，对双阳梅花鹿的两个分支鹿茸的 6 个重要和核心特征进行了标记-性状关联测试。结果表明，与 8 个不同的标记，特别是 M009 和 M027 存在标记-性状关联。
本研究不仅提供了大量的微卫星标记，这对未来的遗传图谱和梅花鹿的性状关联具有重要价值，而且还为梅花鹿的分子育种提供了可用的信息。
