Fleischmann J D, Wentworth D, Valencic F, Imbembo A L, Koehler K A
Surgery Department, Case Western Reserve University School of Medicine, Ohio 44109.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):879-85. doi: 10.1016/s0006-291x(88)80121-9.
The self-association of human recombinant interleukin-2 (IL-2) from E. coli was explored. Self-association, with an apparent Kd of 0.6 micromolar, has pronounced effects on (1) the surface exposure of Trp-121, deduced from quenching studies employing potassium iodide and acrylamide, (2) the apparent quantum yield of Trp-121, the fluorescence of Trp-121 in IL-2 aggregates is 4-fold lower than in IL-2 "monomers", and (3) IL-2-mediated phospholipid vesicle fusion/aggregation.
