Safety and Quality Institute of Agricultural Products,, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.
Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Environ Sci Pollut Res Int. 2020 Oct;27(28):35228-35238. doi: 10.1007/s11356-020-09827-5. Epub 2020 Jun 26.
A quick, easy, cheap, effective, rugged, and safe pretreatment method using dispersive solid-phase extraction was developed to quantify kasugamycin in Chinese cabbage samples by using ultra-performance liquid chromatography/tandem mass spectrometry. A pretreatment method involving precolumn transformation was utilized to determine the residue of saisentong in Chinese cabbage through high-performance liquid chromatography/ultraviolet detection. These methods were successfully applied through field trials to determine the contents of kasugamycin and saisentong in Chinese cabbage. The dissipation of kasugamycin and saisentong in Chinese cabbage followed first-order kinetics with a linear correlation coefficient of 0.9066-0.9731 at the 95% confidence level. The half-lives of kasugamycin and saisentong in Chinese cabbage were 1.8-2.0 and 2.2-3.8 days, respectively. Terminal residual levels of kasugamycin in Chinese cabbage were not detected 14 days after application. The dietary risk assessment of kasugamycin and saisentong in Chinese cabbage showed that their risk quotients were 0.93 and 2.58%, respectively, in the preharvest interval (PHI) of 14 days. Kasugamycin and saisentong in Chinese cabbage did not pose potential health hazards at PHI of 14 days. The maximum residue limits of kasugamycin and saisentong in Chinese cabbage were 0.02 and 0.36 mg/kg, respectively, and 14 days was the safe PHI.
采用快速、简便、廉价、有效、耐用和安全的分散固相萃取前处理方法，结合超高效液相色谱/串联质谱法，建立了同时测定结球白菜中井冈霉素残留量的分析方法。采用柱前衍生化高效液相色谱/紫外检测法，建立了同时测定结球白菜中噻森铜残留量的分析方法。通过田间试验，成功应用上述方法对结球白菜中井冈霉素和噻森铜的含量进行了测定。结果表明，在 95%置信水平下，井冈霉素和噻森铜在结球白菜中的消解均符合一级动力学方程，相关系数分别为 0.9066-0.9731。井冈霉素和噻森铜在结球白菜中的半衰期分别为 1.8-2.0 和 2.2-3.8 d。施药 14 d 后，结球白菜中井冈霉素的最终残留量未检出。结球白菜中井冈霉素和噻森铜的膳食风险评估结果表明，在 14 d 的休药期内，其风险商分别为 0.93 和 2.58%。14 d 的休药期内，结球白菜中井冈霉素和噻森铜对人体健康没有潜在危害。井冈霉素和噻森铜在结球白菜中的最大残留限量分别为 0.02 和 0.36 mg/kg，14 d 为安全休药期。
