Tchervenkov J I, Latter D A, Psychogios J, Christou N V
Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, Canada.
J Trauma. 1988 May;28(5):582-8. doi: 10.1097/00005373-198805000-00003.
This study assessed the effect of burn trauma on the in vivo leukocyte cell delivery during the first 24 hr of the delayed type hypersensitivity (DTH) skin test reaction and a bacterial skin abscess. Inbred male Lewis rats sensitized to keyhole limpet hemocyanin (KLH) were given a 30% scald burn or sham burn. Three days later the animals were injected intradermally, at different sites, with 0.3 mg of KLH, 10(8) organisms of S. aureus 502A, and 0.1 cc of saline, at 2 to 24 hr. Leukocytes labelled with Indium111 oxine(leu111) were injected intravenously. In sham rats the peak leu111 influx in the DTH reaction occurred at 2-4 hr while in the abscess it was biphasic with peaks at 3 hr and 6-8 hr. In burn trauma rats there was a markedly increased leu111 peak at 2 hr in both the DTH and abscess reactions followed by a significantly lower than normal leu111 delivery in the late (6-24) hours. This marked early leukocyte influx in burned rats was paralleled by a reduced DTH skin test lesion (8.2 +/- 1.1 mm to 4.2 +/- 1.1 mm) and an increased bacterial abscess (5.1 +/- 1.1 mm to 8.1 +/- 0.9 mm) post burn. There was a direct correlation between leukocyte cell delivery to a DTH reaction and a bacterial abscess (r8 = 0.69, Spearman rank; p less than 0.001). We conclude that burn trauma results in altered leukocyte delivery to inflammatory lesions and the DTH response can be used to assess the ability of a burn trauma host to recruit leukocytes at a site of infection.
本研究评估了烧伤创伤对迟发型超敏反应（DTH）皮肤试验反应及细菌性皮肤脓肿发生后最初24小时内体内白细胞输送的影响。对钥孔戚血蓝蛋白（KLH）致敏的近交系雄性Lewis大鼠接受30%的烫伤或假烫伤。三天后，在2至24小时内，于不同部位给动物皮内注射0.3毫克KLH、10⁸个金黄色葡萄球菌502A菌株及0.1毫升生理盐水。静脉注射用铟111氧嗪标记的白细胞（leu111）。在假烫伤大鼠中，DTH反应中leu111的峰值流入出现在2 - 4小时，而在脓肿中呈双相，峰值出现在3小时和6 - 8小时。在烧伤创伤大鼠中，DTH和脓肿反应在2小时时leu111峰值均显著增加，随后在后期（6 - 24小时）leu111输送显著低于正常水平。烧伤大鼠中这种明显的早期白细胞流入与烧伤后DTH皮肤试验损伤减小（从8.2±1.1毫米降至4.2±1.1毫米）及细菌性脓肿增大（从5.1±1.1毫米增至8.1±0.9毫米）同时出现。白细胞向DTH反应及细菌性脓肿的输送之间存在直接相关性（r = 0.69，Spearman秩相关；p < 0.001）。我们得出结论，烧伤创伤导致白细胞向炎症病变部位的输送发生改变，DTH反应可用于评估烧伤创伤宿主在感染部位募集白细胞的能力。
