School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
Resources and Environment Innovation Research Institute, School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, PR China.
Bioresour Technol. 2020 Oct;314:123714. doi: 10.1016/j.biortech.2020.123714. Epub 2020 Jun 23.
In the present study, an up-flow solid-phase denitrification biofilm reactor (US-DBR) was established for simultaneous nitrate and dissolved organic matter (DOM) removal from wastewater treatment plant effluent. After 100 days operation, the nitrate and COD removal efficiencies were high of 97% and 80%, respectively. According to EEM-FRI analysis, aromatic and tryptophan protein-like, humic-like and fulvic acid-like substances were identified in DOM. Additionally, protein-like substances in DOM components were much easier transformed as carbon source for denitrification. Moreover, protein secondary structure of DOM changed significantly due to the biodegradation and microorganisms metabolic process. High-throughput sequencing analysis implied that Simplicispira, Diaphorobacter, Hydrogenophaga, Pseudoxanthmonas and Stenotrophomonas were the dominate genera in the whole of US-DBR, that were responsible for the removal of nitrate, organics and degradation of solid carbon source, respectively. This study provided a further biological basis about practical application of solid-phase denitrification for simultaneously remove nitrate and organic matter.
在本研究中，建立了上流式固相反硝化生物膜反应器（US-DBR），用于从污水处理厂出水中同时去除硝酸盐和溶解有机物（DOM）。经过 100 天的运行，硝酸盐和 COD 的去除效率分别高达 97%和 80%。根据 EEM-FRI 分析，在 DOM 中鉴定出芳香族和色氨酸蛋白类、腐殖质类和富里酸类物质。此外，DOM 成分中的蛋白类物质更容易作为反硝化的碳源转化。此外，由于生物降解和微生物代谢过程，DOM 的蛋白质二级结构发生了显著变化。高通量测序分析表明，Simplicispira、Diaphorobacter、Hydrogenophaga、Pseudoxanthmonas 和 Stenotrophomonas 是 US-DBR 中的优势属，分别负责硝酸盐、有机物的去除和固体碳源的降解。本研究为固相反硝化同时去除硝酸盐和有机物的实际应用提供了进一步的生物学依据。
