Department Biologie, Systematische Botanik und Mykologie, GeoBio-Center, Ludwig- Maximilians-Universität München, Menzinger Str. 67, D - 80 638 München, Germany.
Department Biologie, Systematische Botanik und Mykologie, GeoBio-Center, Ludwig- Maximilians-Universität München, Menzinger Str. 67, D - 80 638 München, Germany.
Protist. 2020 Aug;171(4):125741. doi: 10.1016/j.protis.2020.125741. Epub 2020 Jun 3.
Phytodinialean dinophytes are poorly known at present and their phylogenetic relationships largely elusive. Historical names of microscopic species are frequently ambiguous, and a reliable application is impeded although crucial to fully explore the biology of organisms. We collected material close to the type locality of a historical species, namely Dinastridium verrucosum, and established eight strains for morphological and molecular studies. The motile cells showed an obovate shape in outline and were dorso-ventrally slightly flattened. They were orange-brown in colour and had a descending cingulum. In light microscopy, an eyespot was discerned in a few monadoid cells in the central region of the sulcus. Furthermore, a morphologically characteristic, 4-6μm long apical furrow was observed on the episome of the cells in SEM. Older cultivated material further exhibited coccoid cells of irregular shape, with wart-like protuberances and covered by a more or less extensive mucilage. This morphology is indistinguishable from the lectotype of D. verrucosum. In a molecular phylogeny, the species was placed in the Borghiellaceae (†Suessiales). As taxonomic result, we epitypify the historical name, D. verrucosum, and perform the necessary combination to Borghiella.
目前,Phytodinialean 沟鞭藻类的了解程度较低,其系统发育关系也难以捉摸。微观物种的历史名称常常存在歧义,尽管对于充分探索生物的生物学特性至关重要,但可靠的应用受到了阻碍。我们收集了接近历史物种 Dinastridium verrucosum 的模式产地的材料,并建立了 8 个菌株进行形态和分子研究。游动细胞呈长卵形,背腹略扁。它们呈橙棕色,具有下降的环带。在光镜下,在沟的中央区域的少数单细胞中可以分辨出眼点。此外,在 SEM 中,在细胞的周质体上观察到形态特征明显的 4-6μm 长的顶端沟。进一步培养的较老材料进一步表现出不规则形状的球菌细胞,具有疣状突起,并覆盖有或多或少广泛的粘液。这种形态与 D. verrucosum 的模式标本无法区分。在分子系统发育中,该物种被置于 Borghiellaceae(†Suessiales)中。作为分类学结果,我们对历史名称 D. verrucosum 进行了后定型,并对 Borghiella 进行了必要的组合。