Wang Feiyan, Yao Shijie, Cao Duantao, Ju Chao, Yu Sumei, Xu Shiji, Fang Hua, Yu Yunlong
Institute of Pesticide and Environmental Toxicology, the Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Institute of Pesticide and Environmental Toxicology, the Key Laboratory of Molecular Biology of Crop Pathogens and Insects, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
J Hazard Mater. 2020 Dec 5;400:123200. doi: 10.1016/j.jhazmat.2020.123200. Epub 2020 Jun 15.
Triazole-resistance in Aspergillus fumigatus is widespread. We evaluated whether triazole-resistance in A. fumigatus and its related cyp51A mutations, induced by a combination of the triazole fungicides difenoconazole and propiconazole, differs from resistance induced by the individual fungicides. Both difenoconazole and propiconazole can induce triazole-resistance in A. fumigatus. Resistance is much easier induced by formulated fungicides or a combination of these two fungicides compared with standard fungicides or individual fungicides, respectively. Six different mutations (G138S, G138D, H147Y, I246M, M263I and D430N) were identified in the induced resistant strains. The H147Y, I246M and M263I mutations were associated with triazole-resistance. This implies that the application of a combination of difenoconazole and propiconazole may result in higher triazole-resistance in A. fumigatus and more mutations in the cyp51A gene.
烟曲霉对三唑类药物的耐药性广泛存在。我们评估了由三唑类杀菌剂苯醚甲环唑和丙环唑联合诱导产生的烟曲霉对三唑类药物的耐药性及其相关的cyp51A基因突变，是否与由单一杀菌剂诱导产生的耐药性不同。苯醚甲环唑和丙环唑均可诱导烟曲霉产生三唑类耐药性。与标准杀菌剂或单一杀菌剂相比，配方杀菌剂或这两种杀菌剂的组合分别更容易诱导产生耐药性。在诱导产生的耐药菌株中鉴定出六种不同的突变（G138S、G138D、H147Y、I246M、M263I和D430N）。H147Y、I246M和M263I突变与三唑类耐药性相关。这意味着苯醚甲环唑和丙环唑联合使用可能会导致烟曲霉对三唑类药物产生更高的耐药性，并使cyp51A基因发生更多突变。
