Bio-Explore, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, T12 P928, Republic of Ireland.
Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, Northern Ireland.
Arch Virol. 2020 Sep;165(9):2057-2063. doi: 10.1007/s00705-020-04710-7. Epub 2020 Jun 27.
In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic variability was observed between farms at the nucleotide (86.3-95.2%) and amino acid (85.5-96%) levels. Phylogenetic analysis confirmed that all field strains belonged to the European species (type 1) and clustered into three separate groups within the subtype 1 subgroup. This variation may pose challenges for diagnosis and prophylactic control of PRRSV through vaccination in the ROI.
为了研究目前在爱尔兰共和国（ROI）流行的猪繁殖与呼吸综合征病毒（PRRSV）毒株的遗传多样性，对来自四个接种商业畜群的 17 株田间分离株的 ORF5 基因进行了测序和系统进化分析。在核苷酸（86.3-95.2%）和氨基酸（85.5-96%）水平上，农场间的遗传变异性很高。系统进化分析证实，所有田间分离株均属于欧洲种（1 型），并在 1 亚群的亚型 1 内聚集成三个独立的组。这种变异可能会对 ROI 通过疫苗接种诊断和预防控制 PRRSV 带来挑战。
