Quentin-Millet M J, Arminjon F, Danve B, Cadoz M, Armand J
Institut Mérieux, Marcy l'Etoile, Charbonnières, France.
J Biol Stand. 1988 Apr;16(2):99-108. doi: 10.1016/0092-1157(88)90037-6.
An animal model has been developed to assess the safety of acellular pertussis vaccines in terms of reversion to toxicity. Adsorbed pertussis toxoid preparations, alone or combined in a DTP formulation, were administered to nude mice intraperitoneally. In parallel, groups of positive and negative control mice received pertussis toxin and buffer, respectively. The circulating white blood cells of the animals were monitored for 28 days. Mice immunized with glutaraldehyde toxoid preparations did not develop a lymphocytosis during the observation period, whereas mice immunized with an experimental formalin pertussis toxoid vaccine exhibited a high lymphocytosis six days after vaccine administration, demonstrating, in this model, a reversion of the toxoid. The nude mouse model thus appears to reveal the in-vivo reversion of pertussis toxoids and could be included in the quality control panel for the assessment of the safety of acellular pertussis vaccine.
已开发出一种动物模型，用于评估无细胞百日咳疫苗在毒性逆转方面的安全性。将吸附的百日咳类毒素制剂单独或与白喉破伤风联合疫苗（DTP）配方联合，腹腔注射给裸鼠。同时，阳性和阴性对照小鼠组分别接受百日咳毒素和缓冲液。对动物的循环白细胞进行了28天的监测。用戊二醛类毒素制剂免疫的小鼠在观察期内未出现淋巴细胞增多，而用实验性福尔马林百日咳类毒素疫苗免疫的小鼠在疫苗接种后六天出现了高度淋巴细胞增多，在该模型中证明了类毒素的逆转。裸鼠模型因此似乎揭示了百日咳类毒素的体内逆转，可纳入无细胞百日咳疫苗安全性评估的质量控制小组。
