Kokkalas Nikolaos, Kokotis Panagiotis, Diamantopoulou Kalliopi, Galanos Antonios, Lelovas Pavlos, Papachristou Dionysios J, Dontas Ismene A, Triantafyllopoulos Ioannis K
Orthopaedics, Laboratory for Research of the Musculoskeletal System, KAT General Hospital, Medical School, National & Kapodistrian University of Athens, Athens, GRC.
Neurology, Laboratory of Clinical Neurophysiology, Aeginitio Hospital, Medical School, National & Kapodistrian University of Athens, Athens, GRC.
Cureus. 2020 May 24;12(5):e8262. doi: 10.7759/cureus.8262.
Introduction Platelet-rich plasma (PRP) products and mesenchymal stem cells (MSCs) seem to have a significant potential as neurogenic therapeutic modulator systems. This study aimed to investigate such biological blood derivatives that could enhance nerve regeneration when applied locally in the primary repair of peripheral nerve transection of an experimental rat model. Methods A total of 42 two-month-old male Wistar rats were divided into three "treatment" groups (control, PRP, and MSCs). All the subjects were operated under anesthesia, and the surgical site was infiltrated with either normal saline, PRP derived from the animal's peripheral blood, or MSCs derived from the animal's femoral bone marrow. All three groups were also sub-divided into two sub-groups based on the post-operative administration of Non-steroidal anti-inflammatory drugs (NSAIDs) or not in order to evaluate the effect of NSAIDs on the final outcome. Three months post-surgery, electromyography evaluation of both hind limbs (right operated and left non-operated) was performed. The animals were euthanized, and nerve repair specimens were prepared for histology. Results PRP group had a significant effect (p<0.05) on the sciatic nerve repair when compared with the control group, whereas the MSC group had a positive effect but was not statistically significant (p=0.2). The number of counted neural axons at the area distal to the nerve repair site were significantly repetitive (p<0.05) in both the PRP and MSC groups when compared with the control group. Conclusions Both PRP and MSCs appear to play an essential role in the enhancement of nerve repair in terms of functionality and histology. MSCs group demonstrated a positive effect, whereas the PRP group showed statistically significant better results.
引言 富含血小板血浆（PRP）产品和间充质干细胞（MSCs）似乎具有作为神经源性治疗调节系统的巨大潜力。本研究旨在调查此类生物血液衍生物，当局部应用于实验大鼠模型的外周神经横断的初次修复时，是否能够增强神经再生。方法 总共42只两个月大的雄性Wistar大鼠被分为三个“治疗”组（对照组、PRP组和MSCs组）。所有受试者均在麻醉下进行手术，手术部位分别用生理盐水、动物外周血来源的PRP或动物股骨骨髓来源的MSCs浸润。根据术后是否给予非甾体抗炎药（NSAIDs），将所有三组再分为两个亚组，以评估NSAIDs对最终结果的影响。术后三个月，对双后肢（右侧手术侧和左侧未手术侧）进行肌电图评估。处死动物，制备神经修复标本进行组织学检查。结果 与对照组相比，PRP组对坐骨神经修复有显著效果（p<0.05），而MSCs组有积极作用但无统计学意义（p=0.2）。与对照组相比，PRP组和MSCs组在神经修复部位远端区域计数的神经轴突数量均有显著重复性（p<0.05）。结论 PRP和MSCs在功能和组织学方面似乎都在增强神经修复中发挥着重要作用。MSCs组显示出积极作用，而PRP组显示出统计学上显著更好的结果。
