[血管内皮细胞条件培养基对肝癌细胞上皮-间质转化的影响]
[Effect of conditioned medium of vascular endothelial cells on the epithelial-mesenchymal transition of hepatocellular carcinoma cells].
作者信息
Xu Bowen, He Jia, Gao Wenbo, Su Guanyue, Liu Xiaoheng, Shen Yang
机构信息
Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, P.R.China.
出版信息
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2020 Jun 25;37(3):442-449. doi: 10.7507/1001-5515.201907041.
This study aims to investigate the effect of substances secreted or metabolized by vascular endothelial cells on epithelial-mesenchymal transition (EMT) of hepatocellular carcinoma cells under indirect co-culture condition. Human hepatocellular carcinoma cell line QGY-7703 was cultured , and then was co-cultured with conditioned medium of human umbilical vein endothelial cells (HUVEC). The morphological changes of QGY-7703 cells were observed by inverted phase contrast microscopy. The migration ability of QGY-7703 cells was analyzed by scratch-wound assays. The effect of conditioned medium on the expression and distribution of EMT related proteins was detected by Western blot and immunofluorescence assays, respectively. The results showed that the QGY-7703 cells gradually changed from polygonal to spindle shape, the migration ability promoted significantly, and both the expression and distribution of EMT related marker changed in a time-dependent manner after co-culturing. The results confirm that vascular endothelial cells can induce EMT in hepatocellular carcinoma cells under indirect co-culture condition.
本研究旨在探讨在间接共培养条件下，血管内皮细胞分泌或代谢的物质对肝癌细胞上皮-间质转化（EMT）的影响。培养人肝癌细胞系QGY-7703，然后将其与人脐静脉内皮细胞（HUVEC）的条件培养基共培养。通过倒置相差显微镜观察QGY-7703细胞的形态变化。采用划痕试验分析QGY-7703细胞的迁移能力。分别通过蛋白质免疫印迹法和免疫荧光法检测条件培养基对EMT相关蛋白表达和分布的影响。结果显示，共培养后QGY-7703细胞逐渐由多边形变为梭形，迁移能力显著增强，且EMT相关标志物的表达和分布均呈时间依赖性变化。结果证实，在间接共培养条件下，血管内皮细胞可诱导肝癌细胞发生EMT。
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