Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool, UK.
Department of Applied Mathematics, Liverpool John Moores University, Liverpool, UK.
FASEB J. 2020 Aug;34(8):10398-10417. doi: 10.1096/fj.202000668RR. Epub 2020 Jun 29.
Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide ( H O) labeling and chronic low-frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance, and degradation of individual proteins during exercise-induced muscle adaptation. Independent groups of rats received CLFS (10 Hz, 24 h/d) and H O for 0, 10, 20, or 30 days. The extensor digitorum longus (EDL) was isolated from stimulated (Stim) and contralateral non-stimulated (Ctrl) legs. Proteomic analysis encompassed 38 myofibrillar and 46 soluble proteins and the rates of change in abundance, synthesis, and degradation were reported in absolute (ng/d) units. Overall, synthesis and degradation made equal contributions to the adaptation of the proteome, including instances where a decrease in protein-specific degradation primarily accounted for the increase in abundance of the protein.
肌肉对运动的适应是通过改变每个蛋白质的合成或降解来实现的，这是个体蛋白丰度变化的基础。我们使用氘水（ H O）标记和慢性低频刺激（CLFS）在体内研究运动诱导的肌肉适应过程中个体蛋白的合成、丰度和降解。两组大鼠分别接受 CLFS（10 Hz，24 h/d）和 H O 标记 0、10、20 或 30 天。从刺激（Stim）和对侧非刺激（Ctrl）腿中分离出伸趾长肌（EDL）。蛋白质组学分析包括 38 种肌原纤维蛋白和 46 种可溶性蛋白，并以绝对（ng/d）单位报告了丰度、合成和降解的变化率。总的来说，合成和降解对蛋白质组的适应都有贡献，包括在某些情况下，蛋白质特异性降解的减少主要导致蛋白质丰度的增加。
