Gholami Mahnaz D, Theiss Frederick, Sonar Prashant, Ayoko Godwin A, Izake Emad L
Queensland University of Technology (QUT), School of Chemistry and Physics, 2 George street QLD, 4000, Australia.
Analyst. 2020 Aug 10;145(16):5508-5515. doi: 10.1039/d0an00972e.
Recombinant human erythropoietin (rHuEPO) is an important hormone drug that is used to treat several medical conditions. It is also frequently abused by athletes as a performance enhancing agent at sporting events. The time window of the rHuEPO in blood is short. Therefore, the rapid detection of rHuEPO use/abuse at points of care and in sports requires a selective analytical method and a sensitive sensor. Herein, we present a highly selective method for the rapid detection of rHuEPO in human blood plasma by a sensitive optical sensor. rHuEPO is selectively extracted from human blood plasma by a target-specific extractor chip and converted into a biothiol by reducing its disulfide bond structure. The formed biothiol reacts with a water soluble (E)-1-((6-methoxybenzo[d]thiazole-2-yl)diazenyl)naphthalene-2,6-diolHg(ii) (BAN-Hg) optical sensor and causes its rapid decomposition. This leads to a rapid change in the sensor color from blue to pink that can be observed by the naked eye. The optical sensor was used to quantify rHuEPO in the concentration range 1 × 10-8 M to 1 × 10-12 M by UV-Vis spectroscopy. For the screening of blood plasma, an EPO-specific extractor chip was synthesized and used to selectively extract the protein from the biological matrix prior to its conversion into biothiol and quantification by the optical sensor. Since many proteins have a disulfide bond structure, the new method has strong potential for their rapid sensitive and selective detection by the BAN-Hg sensor and UV-Vis spectroscopy.
重组人促红细胞生成素（rHuEPO）是一种重要的激素药物，用于治疗多种病症。它也经常被运动员在体育赛事中作为提高成绩的药物滥用。rHuEPO在血液中的时间窗口很短。因此，在护理点和体育赛事中快速检测rHuEPO的使用/滥用需要一种选择性分析方法和灵敏的传感器。在此，我们展示了一种通过灵敏的光学传感器快速检测人血浆中rHuEPO的高选择性方法。rHuEPO通过目标特异性提取芯片从人血浆中选择性提取，并通过还原其二硫键结构转化为生物硫醇。形成的生物硫醇与水溶性（E）-1-（（6-甲氧基苯并[d]噻唑-2-基）重氮基）萘-2,6-二醇汞（ii）（BAN-Hg）光学传感器反应，并导致其快速分解。这导致传感器颜色从蓝色迅速变为粉红色，肉眼即可观察到。通过紫外-可见光谱法，该光学传感器用于定量浓度范围为1×10-8 M至1×10-12 M的rHuEPO。为了筛选血浆，合成了一种EPO特异性提取芯片，并用于在将蛋白质转化为生物硫醇并通过光学传感器定量之前，从生物基质中选择性提取该蛋白质。由于许多蛋白质具有二硫键结构，这种新方法具有通过BAN-Hg传感器和紫外-可见光谱法对其进行快速灵敏和选择性检测的强大潜力。
