Simon H G, Fruth U, Eckerskorn C, Lottspeich F, Kramer M D, Nerz G, Simon M M
Max-Planck-Institut für Immunobiologie, Freiburg, FRG.
Eur J Immunol. 1988 Jun;18(6):855-61. doi: 10.1002/eji.1830180605.
An oligonucleotide probe corresponding to nucleotides of a cDNA encoding the T cell-associated proteinase 1 (TSP-1) was chosen to study the induction and expression of TSP-1-specific transcripts in mouse T lymphocytes and tissues. We demonstrate that TSP-1 mRNA is only expressed in activated T lymphocytes and is absent from all mouse tissues tested including those containing resting mature T lymphocytes. Expression of the TSP-1 gene was observed in T lymphocytes in vitro in response to either phorbolester (phorbol 12-myristate 13-acetate), Ca2+ ionophore (A23187), lectin or alloantigen. In general, TSP-1 mRNA appeared and peaked later compared to interleukin 2 transcripts. Furthermore, TSP-1 mRNA was inducible in vitro in both Ly-2+ and L3T4+ lymphocyte populations treated with alloantigen and/or lectin. The transcription of the TSP-1 gene was always accompanied by the expression of proteinase activity. High expression of TSP-1 transcripts was also observed in in vivo derived T effector cells specific for lymphocytic choriomeningitis virus. However, TSP-1 mRNA was predominantly associated with virus-specific Ly-2+ T cells and correlated with their proteinase and cytolytic activities. The data suggest that TSP-1 gene transcription is a useful marker to characterize T effector cells in vitro and in vivo.
选择一条与编码T细胞相关蛋白酶1（TSP-1）的cDNA核苷酸相对应的寡核苷酸探针，来研究TSP-1特异性转录本在小鼠T淋巴细胞和组织中的诱导和表达情况。我们证明，TSP-1 mRNA仅在活化的T淋巴细胞中表达，而在所检测的所有小鼠组织中均不存在，包括那些含有静息成熟T淋巴细胞的组织。在体外，T淋巴细胞对佛波酯（佛波醇12-肉豆蔻酸酯13-乙酸酯）、Ca2+离子载体（A23187）、凝集素或同种异体抗原产生反应时，可观察到TSP-1基因的表达。一般来说，与白细胞介素2转录本相比，TSP-1 mRNA出现较晚且峰值也较晚。此外，在用同种异体抗原和/或凝集素处理的Ly-2+和L3T4+淋巴细胞群体中，TSP-1 mRNA在体外是可诱导的。TSP-1基因的转录总是伴随着蛋白酶活性的表达。在体内针对淋巴细胞性脉络丛脑膜炎病毒的T效应细胞中也观察到TSP-1转录本的高表达。然而，TSP-1 mRNA主要与病毒特异性Ly-2+ T细胞相关，并与其蛋白酶和细胞溶解活性相关。这些数据表明，TSP-1基因转录是在体外和体内鉴定T效应细胞的一个有用标志物。
