大鼠的肛门尾骨肌是用于气道上皮衍生舒张因子的便捷生物测定器官。

The rat anococcygeus muscle is a convenient bioassay organ for the airway epithelium-derived relaxant factor.

作者信息

Güc M O, Ilhan M, Kayaalp S O

机构信息

Hacettepe University, Faculty of Medicine, Department of Pharmacology, Ankara, Turkey.

出版信息

Eur J Pharmacol. 1988 Apr 13;148(3):405-9. doi: 10.1016/0014-2999(88)90119-7.

DOI:10.1016/0014-2999(88)90119-7

PMID:3260190

Abstract

The response to epithelium-derived relaxant factor (EpDRF) released from guinea-pig trachea by acetylcholine was studied in the precontracted rat anococcygeus muscle in a coaxial bioassay system. Acetylcholine caused no relaxation of rat anococcygeus muscle which was precontracted with phenylephrine. When the same muscle was placed into a guinea-pig trachea with intact epithelium, acetylcholine produced a slowly developing relaxation which was potentiated by neostigmine and antagonized by atropine but was not altered by indomethacin, propranolol, hydroquinone nor methylene blue. The rat anococcygeus muscle was not relaxed by acetylcholine when it was placed in an epithelium-free trachea. The results indicate that acetylcholine releases EpDRF from guinea-pig trachea and that rat anococcygeus muscle is a convenient organ for the bioassay of EpDRF.

摘要

在同轴生物测定系统中，对预先收缩的大鼠肛门尾骨肌研究了乙酰胆碱从豚鼠气管释放的上皮衍生舒张因子（EpDRF）的反应。用去氧肾上腺素预先收缩的大鼠肛门尾骨肌，乙酰胆碱不会使其舒张。当将同一块肌肉置于具有完整上皮的豚鼠气管中时，乙酰胆碱产生缓慢发展的舒张作用，新斯的明可增强此作用，阿托品可拮抗此作用，但吲哚美辛、普萘洛尔、对苯二酚和亚甲蓝对此无影响。当大鼠肛门尾骨肌置于无上皮的气管中时，乙酰胆碱不会使其舒张。结果表明，乙酰胆碱从豚鼠气管释放EpDRF，并且大鼠肛门尾骨肌是用于EpDRF生物测定的便利器官。