Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
J Basic Microbiol. 2020 Sep;60(9):809-815. doi: 10.1002/jobm.202000186. Epub 2020 Jun 29.
The aim of this study was to select and identify thermophilic bacteria from Caatinga biome (Brazil) able to produce thermoactive keratinases and characterize the keratinase produced by the selected isolate. After enrichment in keratin culture media, an Anoxybacillus caldiproteolyticus PC2 was isolated. This thermotolerant isolate presents a remarkable feature producing a thermostable keratinase at 60°C. The partially purified keratinase, identified as a thermolysin-like peptidase, was active at a pH range of 5.0-10.0 with maximal activity at a temperature range of 50-80°C. The optimal activity was observed at pH 7.0 and 50-60°C. These characteristics are potentially useful for biotechnological purposes such as processing and bioconversion of keratin.
本研究旨在从巴西卡廷加生物群落中筛选和鉴定能够产生热活性角蛋白酶的嗜热细菌，并对所选分离株产生的角蛋白酶进行表征。在角蛋白培养物中进行富集后，分离出一株嗜热的解淀粉芽孢杆菌 PC2。该耐热分离株具有显著的特点，能在 60°C 下产生一种热稳定的角蛋白酶。部分纯化的角蛋白酶被鉴定为一种类似于胰凝乳蛋白酶的肽酶，在 pH 值为 5.0-10.0 的范围内具有活性，在 50-80°C 的温度范围内具有最大活性。最佳活性在 pH 值 7.0 和 50-60°C 下观察到。这些特性在角蛋白加工和生物转化等生物技术方面具有潜在的用途。
