Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, India.
School of Pharmacy, Sungkyunkwan University, Seobu-ro, Jangan-gu, Suwon, Korea.
J Biomol Struct Dyn. 2021 Jul;39(11):4160-4174. doi: 10.1080/07391102.2020.1775124. Epub 2020 Jun 30.
() is a popular invasive seed utilized in the management of diabetes in Jammu and Kashmir, India. The objective of the current study was to scrutinize the antidiabetic effect of (PA) against Streptozotocin (STZ) induced diabetic rats and explore the possible mechanism of action at cellular and sub-cellular levels. Box Benkan Design (BBD) was performed to determine the effect of PA powder to methanol, extraction time and extraction temperature on DPPH and ABTS free radical scavenging activity of decoction. study was performed on GLUT1 (5EQG) and dipeptidyl peptidase IV (DPPIV) (2G63) protein. Type II diabetes mellitus was initiated by single intra-peritoneal injection of STZ. The Blood Glucose Level (BGL) and body weight were estimated at regular interval of time. The different biochemical parameters such as hepatic, antioxidant, and lipid parameters were estimated. At end of the study, pancreas was used for histopathological observation. The variation in DPPH antiradical scavenging activity 40.0-90.0% and ABTS antiradical scavenging activity 34-82%, were estimated respectively. STZ induced DM rats showed increased BGL at end of the experimental study. treatment significantly ( < 0.001) down-regulated the BGL level. significantly ( < 0.001) altered the biochemical, hepatic and antioxidant parameters in a dose-dependent manner. Histopathological examination demonstrated the constructive mass of β-cells in pancreas. Overall, the current study indicates that the treatment down-regulated the hyperglycemic, oxidative stress and hyperlipidaemia in diabetic rats, due to inhibition of enzymes or amelioration of oxidative stress. [Formula: see text] Communicated by Ramaswamy H. Sarma.
在印度查谟和克什米尔地区， （）被广泛用作治疗糖尿病的入侵性种子。本研究的目的是研究 （PA）对链脲佐菌素（STZ）诱导的糖尿病大鼠的降血糖作用，并探讨其在细胞和亚细胞水平的可能作用机制。采用 Box Benkan 设计（BBD）确定 PA 粉对甲醇、提取时间和提取温度对水提物清除 DPPH 和 ABTS 自由基活性的影响。研究了 GLUT1（5EQG）和二肽基肽酶 IV（DPPIV）（2G63）蛋白。通过单次腹腔注射 STZ 诱导 II 型糖尿病。定期测量血糖水平（BGL）和体重。估计了肝、抗氧化和脂质等不同生化参数。研究结束时，用胰腺进行组织病理学观察。分别估计 DPPH 抗自由基清除活性 40.0-90.0%和 ABTS 抗自由基清除活性 34-82%的变化。STZ 诱导的 DM 大鼠在实验研究结束时表现出血糖升高。 治疗显著（ < 0.001）降低了 BGL 水平。 以剂量依赖的方式显著（ < 0.001）改变了生化、肝和抗氧化参数。组织病理学检查显示胰腺β细胞的结构质量良好。总体而言，本研究表明， 治疗通过抑制酶或改善氧化应激，降低了糖尿病大鼠的高血糖、氧化应激和高脂血症。 [公式：见正文]由 Ramaswamy H. Sarma 传达。
