College of Veterinary Medicine, Chungnam National University, Daejeon, Korea.
Department of Clinical Sciences, College of Veterinary and Animal Sciences, Jhang, Sub-campus University of Veterinary and Animal Sciences, Lahore, Jhang, Pakistan.
Reprod Domest Anim. 2020 Sep;55(9):1163-1171. doi: 10.1111/rda.13758. Epub 2020 Jul 18.
Astaxanthin is a member of the carotenoid family well known for its anti-cancer, anti-diabetic, anti-inflammatory and antioxidant nature. This study was designed to investigate the effects of astaxanthin supplementation of the extender (buffer 2) on post-thaw dog semen quality. Semen from four healthy dogs was collected by digital manipulation twice a week. The ejaculates were pooled, washed, divided into four equal aliquots, diluted with the extender supplemented with different concentrations of astaxanthin (0, 0.5, 1 and 2 µM) and cryopreserved. The results showed that 1 µM astaxanthin was the optimum concentration that led to significantly higher (p < .05) post-thaw motility, kinematic parameters and viability than the other groups. In comparison with the control group, sperm samples supplemented with 1 µM astaxanthin showed significantly higher (p < .05) sperm counts with intact membranes (55.7 ± 0.6% vs. 51.3 ± 0.9%), intact acrosome (58.4 ± 0.7% vs. 53.5 ± 0.6%), active mitochondria (54.9 ± 0.5% vs. 42.6 ± 0.6%) and normal chromatin (67.6 ± 0.9% vs. 61.7 ± 0.6%). Furthermore, astaxanthin-supplemented samples showed significantly lower expression levels (p < .05) of pro-apoptotic (BAX), oxidative induced DNA damage repair (OGG1), oxidative stress-related (ROMO1) genes and higher expression levels of anti-apoptotic (BCL2), and sperm acrosome-associated (SPACA3) genes compared to the control. Thus, supplementation of 1 µM astaxanthin in semen extender results in improved freeze-thaw sperm quality of the dog.
虾青素是类胡萝卜素家族的一员，以其抗癌、抗糖尿病、抗炎和抗氧化特性而闻名。本研究旨在探讨在 extender（缓冲液 2）中添加虾青素对解冻后犬精液质量的影响。通过数字操作每周两次从四只健康犬中采集精液。将射精物汇集，洗涤，分成四份等量，用添加不同浓度虾青素（0、0.5、1 和 2µM）的 extender 稀释并冷冻保存。结果表明，1µM 虾青素是最佳浓度，可显著提高（p<0.05）解冻后活力、运动参数和活力。与对照组相比，添加 1µM 虾青素的精子样本显示出更高的（p<0.05）完整膜（55.7±0.6%对 51.3±0.9%）、完整顶体（58.4±0.7%对 53.5±0.6%）、活性线粒体（54.9±0.5%对 42.6±0.6%）和正常染色质（67.6±0.9%对 61.7±0.6%）的精子计数。此外，与对照组相比，虾青素补充组的促凋亡（BAX）、氧化诱导的 DNA 损伤修复（OGG1）、氧化应激相关（ROMO1）基因的表达水平显著降低（p<0.05），而抗凋亡（BCL2）和精子顶体相关（SPACA3）基因的表达水平显著升高。因此，在 semen extender 中添加 1µM 虾青素可提高犬精液的冻融后质量。
