添加槲皮素的乳清蛋白基 extender 可改善公鸡精液的冻后质量。
Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen.
机构信息
Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality, and Security, Ministry of Education, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, 130118, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality, and Security, Ministry of Education, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, 130118, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
出版信息
Cryobiology. 2020 Jun;94:57-65. doi: 10.1016/j.cryobiol.2020.04.010. Epub 2020 May 11.
The advantageous influence of quercetin (Q) supplementation in an extender has not yet been evaluated for rooster semen cryopreservation. This research was purposely conducted in order to assess the effect of different quercetin concentrations added into an extender on the sperm quality of the rooster subsequent to a freezing-thawing process. After the freezing-thawing process, spermatozoa quality parameters (membrane functionality, acrosome integrity, motility, viability, and abnormal morphology), endogenous enzymes (SOD, CAT, and GPx), mitochondrial activity, DNA fragmentation index, lipid peroxidation (MDA), and ROS were all evaluated. A total of 75 neat pooled ejaculates (3 ejaculates/rooster) were collected from 25 arbor acres roosters (24 wks) twice a week using abdominal massage technique, then divided into five equal aliquots and diluted with an extender containing different doses of Q (CS-Q) as follows: casein extender without Q (control only), casein extender containing 0.040 mg/mL quercetin (CS-Q 0.040), 0.020 mg/mL quercetin (CS-Q 0.020), 0.010 mg/mL quercetin (CS-Q 0.010), and 0.005 mg/mL quercetin (CS-Q 0.005). Our results depicted that adding to the extender with a 0.010 mg/mL Q enhanced (P < 0.01) sperm motility, membrane function, viability, mitochondrial activity, intact acrosome (P < 0.05), SOD (P < 0.001), CAT, and GPx (P < 0.01) compared to the control group at post-thaw. Compared to the control group and other treatment groups after the freeze-thawing process, the addition of 0.005 mg/mL Q into the extender also showed higher (P < 0.05) improvement in the quality of sperm parameters and a higher (P < 0.01) SOD and CAT but did not improve mitochondrial activity and sperm viability. In addition, there was a lower degree of DNA fragmentation index, lower (P < 0.05) lipid peroxidation and ROS in frozen-thawed sperm treated with 0.010 mg/mL and 0.005 mg/mL Q than in control and the other treatment groups. In addition, 0.020 mg/mL Q supplementation into the extender also reduced DNA fragmentation and improved GPx activity compared to the control group at post-thaw. Different concentrations of Q 0.010 and 0.005 mg/mL added to the extender reduced the percentage of abnormal spermatozoa compared to the other groups. The results of this study showed for the first time that the inclusion of an extender with a suitable quercetin concentration of 0.010 mg/mL improved the post-thawed quality of rooster semen.
槲皮素（Q）补充剂在 extender 中的有利影响尚未在鸡精液冷冻保存中进行评估。本研究的目的是评估不同浓度的槲皮素添加到 extender 中对冷冻-解冻过程后公鸡精子质量的影响。冷冻-解冻后，评估精子质量参数（膜功能、顶体完整性、运动性、活力和异常形态）、内源性酶（SOD、CAT 和 GPx）、线粒体活性、DNA 碎片化指数、脂质过氧化（MDA）和 ROS。从 25 只 Arbor Acres 公鸡（24 周）中每周两次通过腹部按摩技术收集了 75 个干净的混合精液（每个公鸡 3 个精液），然后将其分成 5 个相等的等分，并用含有不同剂量 Q 的 extender 稀释（CS-Q）如下：不含 Q 的酪蛋白 extender（仅对照）、含 0.040 mg/mL 槲皮素的酪蛋白 extender（CS-Q 0.040）、0.020 mg/mL 槲皮素（CS-Q 0.020）、0.010 mg/mL 槲皮素（CS-Q 0.010）和 0.005 mg/mL 槲皮素（CS-Q 0.005）。我们的结果表明，与对照组相比，在解冻后，添加 0.010 mg/mL Q 的 extender 可提高（P<0.01）精子运动性、膜功能、活力、线粒体活性、完整顶体（P<0.05）、SOD（P<0.001）、CAT 和 GPx（P<0.01）。与对照组和其他处理组相比，在冷冻-解冻过程后，在 extender 中添加 0.005 mg/mL Q 也可以提高（P<0.05）精子参数的质量，并提高（P<0.01）SOD 和 CAT，但不能提高线粒体活性和精子活力。此外，在冷冻和解冻的精子中，添加 0.010 和 0.005 mg/mL Q 的处理组的 DNA 碎片化指数、脂质过氧化和 ROS 水平均低于对照组和其他处理组（P<0.05）。此外，与对照组相比，CS-Q 0.020 补充剂也可以降低 DNA 碎片化并提高解冻后的 GPx 活性。添加到 extender 中的不同浓度 Q（0.010 和 0.005 mg/mL）与其他组相比降低了异常精子的百分比。本研究首次表明，在 extender 中添加合适浓度的槲皮素（0.010 mg/mL）可提高公鸡精液的解冻后质量。
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