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[黑斑蛙从受精到第一次卵裂期胚胎发育对孵化条件的依赖性]

[Dependence of the embryonic development of Rana temporaria on incubation conditions in the period from insemination to the first cleavage division].

作者信息

Sharova L V, Bozhkova V P

出版信息

Ontogenez. 1988 Mar-Apr;19(2):149-55.

PMID:3260365
Abstract

The R. temporaria embryos at stages from insemination to the 1st cleavage division were incubated for 1 tau 0 in different saline solutions. The subsequent development in the control and after all kinds of treatment proceeded in the same conditions (in settled tap water and at 19-20 degrees). After incubation in the solutions containing potassium and sodium chlorides, bromides or iodides in a concentration of 30 mM, which decreased the amplitude of activation potential and the value of transmembrane currents, the morphogenetic movement during neurulation and, more rarely, gastrulation were accelerated (coefficient of relative velocity 1.16-1.38). After treatment with calcium chloride, gluconate and choline chloride in the same concentration, the morphogenetic processes were delayed (0.85-0.95).

摘要

将欧洲林蛙从受精到第一次卵裂期的胚胎在不同盐溶液中孵育1τ0。对照组以及所有处理后的后续发育均在相同条件下进行(在静置的自来水中,温度为19 - 20摄氏度)。在含有浓度为30 mM的氯化钾、溴化钾或碘化钾的溶液中孵育后,激活电位的幅度和跨膜电流值降低,神经胚形成期间的形态发生运动,更罕见的是原肠胚形成加速(相对速度系数为1.16 - 1.38)。用相同浓度的氯化钙、葡萄糖酸盐和氯化胆碱处理后,形态发生过程延迟(0.85 - 0.95)。

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