Food Technology Department, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Department of Biochemistry, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt.
Int J Biol Macromol. 2020 Nov 1;162:1195-1203. doi: 10.1016/j.ijbiomac.2020.06.243. Epub 2020 Jun 27.
Findings small molecules with protein disaggregation effects are lately needed. For the first time, we studied the in vitro-antifibrillogenic effects of cyanidin 3-rutinoside (C3R), purified from mulberry fruits, on bovine serum albumin (BSA) under aggregation-promoting conditions, using multispectral, microstructure, and molecular docking approaches. Results showed that C3R dose-dependently inhibited BSA-aggregations under the glycation conditions through separating the size peak, influencing Trp-intensity and hydrophobicity, affecting cross-β-sheet conformations, and microstructural declining the aggregates of glycated-BSA. Throughout the underlying mechanism behind the disaggregation effects, C3R altered the secondary structure, SDS-PAGE-bands, and XRD-peaks of glycated-BSA aggregates, as well as interacted with some of lysyl and arginine (Lys114, Lys431, Arg427, and Arg185) glycation sites of BSA. Overall, these results unleash that monomeric anthocyanins restrict BSA-aggregations under the glycation conditions which can assist in the design of reasonable therapeutics and functional foods.
最近需要寻找具有蛋白解聚作用的小分子。我们首次使用多光谱、微观结构和分子对接方法，研究了从桑椹中纯化得到的矢车菊素-3-芸香糖苷（C3R）在促聚集条件下对牛血清白蛋白（BSA）的体外抗纤维化作用。结果表明，C3R 在糖基化条件下通过分离大小峰、影响色氨酸强度和疏水性、影响交叉-β-折叠构象以及降低糖基化-BSA 聚集物的微观结构，剂量依赖性地抑制 BSA 聚集。通过研究解聚作用的潜在机制，C3R 改变了糖基化 BSA 聚集物的二级结构、SDS-PAGE 带和 XRD 峰，以及与 BSA 的一些赖氨酸和精氨酸（Lys114、Lys431、Arg427 和 Arg185）糖基化位点相互作用。总的来说，这些结果表明单体类黄酮在糖基化条件下抑制 BSA 聚集，这可以有助于设计合理的治疗方法和功能性食品。
