Thilavech Thavaree, Ngamukote Sathaporn, Abeywardena Mahinda, Adisakwattana Sirichai
Program in Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand; Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok 10330, Thailand; Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Biol Macromol. 2015 Apr;75:515-20. doi: 10.1016/j.ijbiomac.2015.02.004. Epub 2015 Feb 12.
Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, is present in various fruits and vegetables as a colorant. C3R has been well characterized and demonstrated a number of biological activities attributed to its antioxidant properties. The present study compared the effectiveness of C3R against monosaccharide-induced protein glycation and oxidation in vitro using bovine serum albumin (BSA).The results demonstrated that C3R (0.125-1.00 mM) inhibited the formation of fluorescent AGEs in ribose-glycated BSA (2-52%), fructose-glycated BSA (81-93%), glucose-glycated BSA (30-74%) and galactose-glycated BSA (6-79%).Correspondingly, C3R (1.00 mM) decreased the level of N(ɛ)-(carboxymethyl) lysine (56-86%) in monosaccharide-induced glycation in BSA. C3R also reduced the level of fructosamine, β-amyloid cross structure, protein carbonyl content as well as the depletion of thiol in BSA/monosaccharide system. In summary, C3R might offer a new promising antiglycation agent for the prevention of diabetic complications by inhibiting AGE formation and oxidation-dependent protein damage.
矢车菊素 - 3 - 芸香糖苷（C3R）是一种天然存在的花青素，作为一种色素存在于各种水果和蔬菜中。C3R已得到充分表征，并因其抗氧化特性而具有多种生物活性。本研究使用牛血清白蛋白（BSA）比较了C3R在体外对抗单糖诱导的蛋白质糖基化和氧化的效果。结果表明，C3R（0.125 - 1.00 mM）可抑制核糖糖化BSA（2 - 52%）、果糖糖化BSA（81 - 93%）、葡萄糖糖化BSA（30 - 74%）和半乳糖糖化BSA（6 - 79%）中荧光晚期糖基化终产物的形成。相应地，C3R（1.00 mM）可降低BSA中单糖诱导糖基化过程中N（ɛ） - （羧甲基）赖氨酸的水平（56 - 86%）。C3R还降低了BSA/单糖体系中果糖胺水平、β - 淀粉样蛋白交叉结构、蛋白质羰基含量以及巯基的消耗。总之，C3R可能通过抑制晚期糖基化终产物的形成和氧化依赖性蛋白质损伤，为预防糖尿病并发症提供一种新的有前景的抗糖基化剂。
