Tang Yan, Zheng Xiaodong, Liu Haijuan, Sunxie Feige
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China.
Genomics. 2020 Nov;112(6):3893-3902. doi: 10.1016/j.ygeno.2020.06.036. Epub 2020 Jun 27.
This study presented 96 cox1 and 76 cox3 genes of Amphioctopus neglectus populations. Three distinct lineages were formed from phylogenetic trees and networks constructed using haplotypes. Mitogenomes of A. neglectus-a and A. neglectus-b as the representatives of two lineages separated from population genetics were sequenced to compare with A. neglectus at the genome-level. Amphioctopus neglectus-a showed significant differences with A. neglectus, mainly reflected in gene length, intergenic regions and the secondary structure of tandem repeat motifs. Notably, two sequence deletions in mitogenomes of the two representative species were detected in different positions of major non-coding regions, which were the most distinct differences with A. neglectus. Pairwise genetic distances and the phylogenetic analysis supported the relationship of (A. neglectus-a + (A. neglectus + A. neglectus-b)). This study suggested that A. neglectus-a should be considered as a potential cryptic species of this complex, while A. neglectus-b needed further verification to be defined.
本研究展示了疏忽双盘耳乌贼种群的96个细胞色素氧化酶亚基1（cox1）基因和76个细胞色素氧化酶亚基3（cox3）基因。利用单倍型构建的系统发育树和网络形成了三个不同的谱系。对作为从群体遗传学中分离出的两个谱系代表的疏忽双盘耳乌贼-a和疏忽双盘耳乌贼-b的线粒体基因组进行了测序，以便在基因组水平上与疏忽双盘耳乌贼进行比较。疏忽双盘耳乌贼-a与疏忽双盘耳乌贼表现出显著差异，主要体现在基因长度、基因间隔区和串联重复基序的二级结构上。值得注意的是，在两个代表性物种的线粒体基因组中检测到的两个序列缺失位于主要非编码区的不同位置，这是与疏忽双盘耳乌贼最明显的差异。成对遗传距离和系统发育分析支持（疏忽双盘耳乌贼-a +（疏忽双盘耳乌贼 + 疏忽双盘耳乌贼-b））的关系。本研究表明，疏忽双盘耳乌贼-a应被视为该复合体的一个潜在隐存种，而疏忽双盘耳乌贼-b需要进一步验证才能确定。
