Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey.
Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey.
Diagn Microbiol Infect Dis. 2020 Sep;98(1):115098. doi: 10.1016/j.diagmicrobio.2020.115098. Epub 2020 Jun 1.
Escherichia coli ST131 clone and H30-R/H30-Rx subclones are the most common multidrug-resistant high-risk clones in UTIs. Antimicrobial susceptibility of fosfomycin was compared to five other agents in consecutively collected 299 urinary isolates using the agar dilution method. Prevalence of the ST131 clone and the occurrence of blaCTX-M were also investigated. Overall resistance to fosfomycin, cefuroxime, and ceftriaxone were 2.7%, 35.4%, and 30.1% respectively. fosA, fosA3, and fosC2 genes were not detected. In isolates resistant to ciprofloxacin (34.7%), the prevalence of ST131 clone was 31.7%, of which 81.8% belonged to H30-R and 66.7% to H30-Rx subclones. None of the isolates of the ST131 clone were resistant to fosfomycin. However, bla occurred in 57.6% of the isolates among this clone, 62.9% in H30-R and 68.2% in H30-Rx subclones. The results of this study suggest that fosfomycin resistance is not prevalent in urinary isolates, however, blapositive ST131 clone is quite common.
产酸克雷伯菌 ST131 克隆和 H30-R/H30-Rx 亚克隆是尿路感染中最常见的多药耐药高风险克隆。采用琼脂稀释法比较了连续收集的 299 份尿分离株中磷霉素与其他 5 种药物的药敏性。还调查了 ST131 克隆的流行情况和 blaCTX-M 的发生情况。对磷霉素、头孢呋辛和头孢曲松的总体耐药率分别为 2.7%、35.4%和 30.1%。未检测到 fosA、fosA3 和 fosC2 基因。在对环丙沙星耐药的 34.7%的分离株中，ST131 克隆的流行率为 31.7%，其中 81.8%属于 H30-R 亚克隆，66.7%属于 H30-Rx 亚克隆。ST131 克隆的分离株均未对磷霉素产生耐药性。然而，该克隆中 57.6%的分离株携带 blaCTX-M，其中 H30-R 亚克隆为 62.9%，H30-Rx 亚克隆为 68.2%。本研究结果表明，尿分离株中磷霉素耐药性并不普遍，但 blaCTX-M 阳性的 ST131 克隆相当常见。
