Nano Aggregate Formation Through Self-Assembly of Poly(L-lysine)-Block-Poly(-benzyl-L-glutamate)-Graft-Poly(ethylene glycol) Copolymer.

Polymeric nano aggregates based on poly(-lysine)--PBLG--poly(ethylene glycol) (PLL--PBLG--PEG) copolymers were prepared by a dialysis method in deionized water. PLL--PBLG--PEG copolymers with different degrees of PEG substitution were synthesized by combining the ring-opening polymerization of -amino acid -carboxyanhydrides and ester exchange reactions. Proton nuclear magnetic resonance spectroscopy and gel permeation chromatography were employed to confirm the polymer structures and molecular weights. Transmission electron microscopy and dynamic light scattering were used to observe the self-assembly behavior. The experimental results revealed that the volume content of deionized water in the mixture significantly affects the morphology and size of the aggregates formed by PLL--PBLG--PEG copolymers. Spherical, spindle-like, needle-like, and daisy-like aggregates were observed with an increase in the volume content of deionized water in the mixture.