佛波酯调节5-羟色胺受体介导的培养大鼠血管平滑肌细胞中肌醇磷酸生成和钙动员的增加。

Phorbol ester modulates serotonin receptor-mediated increases in inositol phosphate production and calcium mobilization in cultured rat vascular smooth muscle cells.

作者信息

Takata S, Hirata Y, Takagi Y, Yoshimi H, Fukuda Y, Fujita T

机构信息

Hyptertension-Endocrine Division, National Cardiovascular Center Research Institute, Osaka, Japan.

出版信息

FEBS Lett. 1988 Jul 4;234(1):228-30. doi: 10.1016/0014-5793(88)81340-1.

DOI:10.1016/0014-5793(88)81340-1

PMID:3260561

Abstract

The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on serotonin-induced inositol phosphate (IP) accumulation and intracellular free Ca2+ concentrations [( Ca2+]i) was investigated in cultured rat vascular smooth muscle cells. Pretreatment with TPA had no effect on basal levels of both IP production and [Ca2+]i, whereas it significantly attenuated serotonin-induced increases in both IP production and [Ca2+]i. These data suggest that protein kinase C is involved in the negative feedback control of serotonin-induced rises in both IP production and [Ca2+]i.

摘要

研究了12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对培养的大鼠血管平滑肌细胞中5 - 羟色胺诱导的肌醇磷酸（IP）积累和细胞内游离钙离子浓度[Ca2+]i的影响。TPA预处理对IP生成和[Ca2+]i的基础水平无影响，而它显著减弱了5 - 羟色胺诱导的IP生成和[Ca2+]i的增加。这些数据表明蛋白激酶C参与了5 - 羟色胺诱导的IP生成和[Ca2+]i升高的负反馈控制。