MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Vet Microbiol. 2020 Jul;246:108728. doi: 10.1016/j.vetmic.2020.108728. Epub 2020 May 24.
Sporadic outbreaks of Seneca Valley virus (SVV) have been detected in recent years causing huge economic losses to the pig industry. SVV infection can lead to redness and fever of the mouth, nose or hoof wall, ulcerative injury and inflammation in pigs. Although long non-coding RNAs (lncRNAs) have been shown to play an important role in antiviral and inflammatory regulation, how lncRNAs regulate and induce SVV infection inflammation remains unclear. Here, we found the differential expression of 1332 lncRNAs and 3299 mRNAs in SVV-infected ST cells using RNA-seq. Functional annotation analysis revealed that regulated transcripts are mainly involved in signaling pathways related to host immunity and inflammatory responses. We identified lnc-MSTRG.18940.1 as an important immune regulator in SVV infection. Lnc-MSTRG.18940.1 silencing specifically inhibited SVV replication and the production of inflammatory factors TNF-α, IL-1, IL-6, and IL-8. Our findings aid to a better understanding of host responses to SVV infection and provide new directions for understanding the potential association between lncRNAs and SVV pathogenesis.
近年来，塞内卡谷病毒（SVV）的零星爆发已被检测到，给养猪业造成了巨大的经济损失。SVV 感染可导致猪的口腔、鼻腔或蹄壁发红、发热、溃疡性损伤和炎症。尽管长链非编码 RNA（lncRNA）已被证明在抗病毒和炎症调节中发挥重要作用，但 lncRNA 如何调节和诱导 SVV 感染炎症仍不清楚。在这里，我们使用 RNA-seq 发现 SVV 感染 ST 细胞中 1332 个 lncRNA 和 3299 个 mRNA 的差异表达。功能注释分析表明，受调控的转录本主要参与与宿主免疫和炎症反应相关的信号通路。我们确定 lnc-MSTRG.18940.1 是 SVV 感染中的一个重要免疫调节因子。lnc-MSTRG.18940.1 的沉默特异性抑制了 SVV 的复制和炎症因子 TNF-α、IL-1、IL-6 和 IL-8 的产生。我们的研究结果有助于更好地理解宿主对 SVV 感染的反应，并为理解 lncRNA 与 SVV 发病机制之间的潜在关联提供了新的方向。
