Hahn A F, Feasby T E, Steele A, Lovgren D S, Berry J
Department of Clinical Neurological Sciences, University of Western Ontario, London, Canada.
Lab Invest. 1988 Jul;59(1):115-25.
We describe the clinical and pathologic features of experimental allergic neuritis in Lewis rats inoculated with varying doses of myelin, ranging from 0.5 to 20 mg. The clinical scores were assessed daily. On days 18 and 19 or 28 postimmunization, the rats were perfused with fixative and samples of cervical and lumbar roots and sciatic nerves were processed and embedded in Epon. Tissues were examined by light and electron microscopy and the degree of edema, inflammation, demyelination, and axonal degeneration was assessed quantitatively. We found that the severity of clinical and pathologic experimental allergic neuritis correlated positively with the dose of myelin used for immunization. High dose tolerance was not observed. Demyelination prevailed in nerve roots and increased with higher doses of antigen. Accompanying axonal degeneration was seen only with high doses of myelin. The pathology of sciatic nerves differed. Sciatic nerves of rats immunized with 0.5 and 1 mg of myelin were either normal or showed perivenular lymphocytic infiltrates and demyelination, but nerves from rats with higher immunizing doses of myelin showed increasingly severe axonal degeneration. The axonal degeneration in nerve roots paralleled the degree of inflammation and demyelination and may have been a nonspecific product of the inflammatory reaction. However, the much more severe axonal destruction seen in sciatic nerves with high myelin doses was probably due to other pathogenetic mechanisms.
我们描述了用0.5至20毫克不同剂量髓磷脂接种的Lewis大鼠实验性变应性神经炎的临床和病理特征。每天评估临床评分。在免疫后第18天和第19天或第28天，用固定剂灌注大鼠，处理颈神经根、腰神经根和坐骨神经样本，并包埋于环氧树脂中。通过光镜和电镜检查组织，并定量评估水肿、炎症、脱髓鞘和轴索变性的程度。我们发现，实验性变应性神经炎的临床和病理严重程度与用于免疫的髓磷脂剂量呈正相关。未观察到高剂量耐受性。神经根中脱髓鞘占主导，且随着抗原剂量增加而加重。仅在高剂量髓磷脂时可见伴随的轴索变性。坐骨神经的病理情况有所不同。用0.5毫克和1毫克髓磷脂免疫的大鼠坐骨神经正常或表现为血管周围淋巴细胞浸润和脱髓鞘，但用更高免疫剂量髓磷脂免疫的大鼠的神经显示出越来越严重的轴索变性。神经根中的轴索变性与炎症和脱髓鞘程度平行，可能是炎症反应的非特异性产物。然而，高剂量髓磷脂的坐骨神经中所见的更严重的轴索破坏可能是由于其他致病机制。
