Kupffer Cell and Hepatocyte Isolation from a Single Mouse Liver by Gradient Centrifugation.

Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we describe a methodology to isolate both Kupffer cells and hepatocytes from a single mouse, which provides the unique advantage of studying these two cell types from the same liver.