Sorbonne-Université, Paris Cedex 05, France.
Institut Jean-Pierre Bourgin, Equipe VAST, Versailles Cedex, France.
Methods Mol Biol. 2020;2156:61-84. doi: 10.1007/978-1-0716-0660-5_7.
Most agronomic traits are determined by quantitative trait loci (QTL) and exhibit continuous distribution in natural or especially built segregating populations. The genetic architecture and the hereditary characteristics of these traits are much more complicated than those of oligogenic traits and need adapted strategies for deciphering. The model plant Arabidopsis thaliana is widely studied for quantitative traits, especially via the utilization of genetic natural diversity. Here we describe a QTL-mapping protocol for analyzing freezing tolerance after cold acclimation in this species, based on its specific genetic tools. Nevertheless, this approach can be applied for the elucidation of complex traits in others species.
大多数农艺性状由数量性状位点（QTL）决定，并在自然或特别构建的分离群体中表现出连续分布。这些性状的遗传结构和遗传特征比寡基因性状复杂得多，需要适应的策略来破译。模式植物拟南芥被广泛研究用于数量性状，特别是通过利用遗传自然多样性。在这里，我们描述了一种基于其特定遗传工具分析该物种冷驯化后耐寒性的 QTL 作图协议。然而，这种方法也可以应用于其他物种复杂性状的阐明。
