Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Forestry and Forest Products Research Institute, Forest Tree Breeding Center, Hitachi, Japan.
Methods Mol Biol. 2020;2156:99-117. doi: 10.1007/978-1-0716-0660-5_9.
A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, the essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.
一台冷冻扫描电子显微镜（cryo-SEM）是观察大块冷冻样本的一种很有价值的工具，可以监测植物组织和细胞的冷冻反应。这里，我们描述了 cryo-SEM 观察植物组织细胞冷冻行为的基本过程。
