Wang Xiao-Tong, Yang Hong, Su Yang, Liu Xu-Yan
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.
Huan Jing Ke Xue. 2020 Feb 8;41(2):839-848. doi: 10.13227/j.hjkx.201908157.
To solve the problems of a slow start, easy loss, and easily inhibited activity of the Anammox bacteria suspension culture, polyvinyl alcohol-polypropylene (PVA-PP) was used to prepare the Anammox immobilized filler. To improve the activity of Anammox bacteria and ensure stable operation of the reaction system, the effects of COD interference, change in pH value, and rotating speed on the nitrogen removal characteristics of the immobilized filler were determined in batch tests. Changes in the structure and diversity of the bacteria in the filler were analyzed by a high-throughput sequencing technique. The results showed that the activity of Anammox bacteria could recover to 100% on the 30th day, and the total nitrogen removal rate was 87.7% when the total nitrogen volume load (NLR) was 0.69 kg·(m·d) at the stage of 99 days. After 140 days of long-term operation, the total nitrogen removal rate (NRR) reached 1.83 kg·(m·d), which was 9.4 times the suspended sludge before immobilization. The diversity of the population was maintained in the inclusion carrier, and the effective enrichment of (AF375995.1), which performs anaerobic ammonia-oxidization, increased from 11.06% to 32.55%. The influence of COD interference and changes in the pH value of Anammox bacteria was significantly weakened, and the PVA-PP entrapped carrier could achieve the coupling removal of nitrogen by Anammox and denitrification. Appropriate external hydraulic disturbance would promote the Anammox reaction in immobilized systems.
为解决厌氧氨氧化菌悬浮培养启动慢、易流失、活性易受抑制等问题，采用聚乙烯醇 - 聚丙烯（PVA - PP）制备厌氧氨氧化固定化填料。为提高厌氧氨氧化菌活性并确保反应系统稳定运行，通过批次试验确定了化学需氧量（COD）干扰、pH值变化和转速对固定化填料脱氮特性的影响。采用高通量测序技术分析了填料中细菌的结构和多样性变化。结果表明，厌氧氨氧化菌活性在第30天可恢复至100%，在99天阶段总氮容积负荷（NLR）为0.69 kg·(m³·d)时，总氮去除率为87.7%。经过140天长期运行，总氮去除速率（NRR）达到1.83 kg·(m³·d)，是固定化前悬浮污泥的9.4倍。在包埋载体中保持了菌群的多样性，进行厌氧氨氧化的（AF375995.1）有效富集率从11.06%提高到32.55%。COD干扰和pH值变化对厌氧氨氧化菌的影响显著减弱，PVA - PP包埋载体可实现厌氧氨氧化与反硝化耦合脱氮。适当的外部水力扰动会促进固定化系统中的厌氧氨氧化反应。
