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[畜禽粪便与桃枝工业堆肥过程中微生物群落演替及其与环境因子的关系]

[Microbial Community Succession in Industrial Composting with Livestock Manure and Peach Branches and Relations with Environmental Factors].

作者信息

Cai Han-Bing, Feng Wen-Wen, Dong Yong-Hua, Ma Zhong-Liang, Cao Hui-Jin, Sun Jun-Song, Zhang Bao-Guo

机构信息

School of Life Sciences, Shanghai University, Shanghai 200444, China.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.

出版信息

Huan Jing Ke Xue. 2020 Feb 8;41(2):997-1004. doi: 10.13227/j.hjkx.201907153.

Abstract

This study sets out to understand the evolution of the microbial community structure in industrial composting with livestock manure and peach branches. Pig manure, peach branches, and decomposed organic fertilizer were used as materials for composting. Changes in physical and chemical indicators and the evolution in the structure of the compost microbial community, determined by high-throughput sequencing, were analyzed. The results of physical and chemical parameters show that the pile reached the high-temperature stage on day 2, and the thermophilic period lasted for 30 days. The changes in total carbon were volatile, and there was an overall decline in the amount of TOC in the whole process of composting; The final content of TN was 20.58 g·kg, which was 5.90% lower compared to the initial compost. Alpha analysis indicated that a different microbial community diversity existed at different times during aerobic composting periods. At the bacterial phyla level, Firmicutes and Actinobacteria were the dominant phyla, and the proportion of relative abundance were 79.31%-95.09% and 2.98%-19.70%, respectively, in the entire compost. The relative abundance of Firmicutes and Actinobacteria were 87.36% and 9.66%, respectively, and their respective relative abundances were 79.38% and 19.70% at the end of composting. At the bacterial genus level, the dominant group changed from , , , and ; Regarding the fungus phyla, the Ascomycota was the dominant phylum. For the fungus genus, the relative abundance of gradually increased during composting, and finally was predominant group. The redundancy analysis (RDA) showed that the correlation rank between environmental factors and microbial community structure was:pH > NH-N > > TOC > TN, where pH had the greatest impact on the microbial community composition. , , and may be related to the volatilization of ammonium nitrogen.

摘要

本研究旨在了解以畜禽粪便和桃树枝为原料进行工业堆肥过程中微生物群落结构的演变。以猪粪、桃树枝和腐熟有机肥作为堆肥材料。分析了理化指标的变化以及通过高通量测序确定的堆肥微生物群落结构的演变。理化参数结果表明,堆体在第2天达到高温阶段,嗜热期持续30天。总碳变化不稳定,堆肥全过程TOC含量总体呈下降趋势;TN最终含量为20.58 g·kg,相较于堆肥初始值降低了5.90%。Alpha分析表明,好氧堆肥期间不同时间存在不同的微生物群落多样性。在细菌门水平上,厚壁菌门和放线菌门是优势菌门,在整个堆肥过程中相对丰度比例分别为79.31% - 95.09%和2.98% - 19.70%。厚壁菌门和放线菌门的相对丰度分别为87.36%和9.66%,在堆肥结束时其相对丰度分别为79.38%和19.70%。在细菌属水平上,优势菌群从……变为……;关于真菌门,子囊菌门是优势菌门。对于真菌属,……在堆肥过程中相对丰度逐渐增加,最终成为优势菌群。冗余分析(RDA)表明,环境因素与微生物群落结构的相关排序为:pH > NH₄⁺-N >……> TOC > TN,其中pH对微生物群落组成影响最大。……和……可能与铵态氮的挥发有关。

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