Berchuck A, MacDonald P C, Milewich L, Casey M L
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical School, Dallas 75235.
Mol Cell Endocrinol. 1988 May;57(1-2):87-92. doi: 10.1016/0303-7207(88)90036-6.
The cell line designated as A431 is characterized by an extraordinary capacity for binding of epidermal growth factor (EGF); but paradoxically, these cells also are characterized by failure of replication in response to treatment with EGF and other mitogens. In this study, we took advantage of this unique response of A431 cells to EGF and other mitogenic agents. Specifically, we investigated the dependency of mitogen-stimulated prostaglandin production on mitogenesis. The effects of treatment of A431 cells with epidermal growth factor and two other mitogenic agents, viz., sodium orthovanadate and 12-O-tetradecanoylphorbol-13-acetate (TPA), on DNA synthesis and prostaglandin production were evaluated. The rate of prostaglandin production in A431 cells that were treated with each of these agents increased in a manner that was dependent upon the duration of treatment and the concentration of the agent tested. Thus, by use of A431 cells, we find that the effects of 'mitogenic' agents on cell replication and prostaglandin production are clearly dissociable.
名为A431的细胞系具有非凡的表皮生长因子（EGF）结合能力；但自相矛盾的是，这些细胞的特征还在于，在用EGF和其他促有丝分裂原处理时无法进行复制。在本研究中，我们利用了A431细胞对EGF和其他促有丝分裂剂的这种独特反应。具体而言，我们研究了促有丝分裂原刺激的前列腺素产生对有丝分裂的依赖性。评估了用表皮生长因子以及其他两种促有丝分裂剂（即原钒酸钠和12-O-十四烷酰佛波醇-13-乙酸酯（TPA））处理A431细胞对DNA合成和前列腺素产生的影响。用这些试剂中的每一种处理的A431细胞中前列腺素的产生速率以取决于处理持续时间和所测试试剂浓度的方式增加。因此，通过使用A431细胞，我们发现“促有丝分裂”剂对细胞复制和前列腺素产生的影响明显是可分离的。
