Xue Jia-Jun, Zhang Shao-Qing, Zhang Li-Qiu, Li Shu-Geng, Yao Hai-Nan, Geng Zhong-Xuan, Li Hong, Liu Xiao-Ling
School of Civil Engineering, Guangzhou University, Guangzhou 510006, China.
Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.
Huan Jing Ke Xue. 2020 Jun 8;41(6):2796-2804. doi: 10.13227/j.hjkx.201911134.
A SNAD(simultaneous partial nitrification,ANAMMOX,and denitrification) process initiated in a submerged biological aerated filter (SBAF) was started up by seeding nitrification sludge and an ANAMMOX filter to investigate the operating characteristics and the succession of functional bacteria. The results showed that when the autotrophic nitrogen removal and denitrification were operated stably for 67 days at an initial COD concentration of 60 mg·L, the maximum nitrogen removal efficiency, the COD removal rate, and the nitrogen removal rate were 92.0%, 82.9%, and 2.3 kg·(m·d), respectively. Moreover, the total nitrogen removal rate of the SNAD process in this study was 12.6% higher than that of CANON process. The results of quantitative PCR showed that the abundance of AOB slightly increased, while the abundance of ANAMMOX was one order higher than that before the start-up of SNAD. In comparison, the abundances of denitrifiers and NOB remained at a relatively low level (10 copies·g). Taken together, these results suggested that the SBAF process with volcanic filter was beneficial for the accumulation of ANAMMOX and AOB.
通过接种硝化污泥和厌氧氨氧化滤池,启动了在淹没式曝气生物滤池(SBAF)中进行的同时亚硝化、厌氧氨氧化和反硝化(SNAD)工艺,以研究其运行特性和功能细菌的演替。结果表明,当初始化学需氧量(COD)浓度为60mg·L时,自养脱氮和反硝化稳定运行67天,最大脱氮效率、COD去除率和氮去除率分别为92.0%、82.9%和2.3kg·(m³·d)。此外,本研究中SNAD工艺的总氮去除率比全程自养脱氮工艺(CANON)高12.6%。定量聚合酶链反应(PCR)结果表明,氨氧化细菌(AOB)的丰度略有增加,而厌氧氨氧化菌的丰度比SNAD启动前高一个数量级。相比之下,反硝化菌和亚硝酸盐氧化菌(NOB)的丰度保持在相对较低的水平(10⁸拷贝·g)。综上所述,这些结果表明带有火山滤料的SBAF工艺有利于厌氧氨氧化菌和氨氧化细菌的富集。