Medical Research Center, Peking University Third Hospital, Beijing 100191, China.
Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
Aging (Albany NY). 2020 Jun 30;12(13):12987-13004. doi: 10.18632/aging.103385.
Circulating miRNAs have received extensive attention as non-invasive biomarkers for prediction and diagnosis of disease. However, most samples have been obtained from peripheral venous blood. To evaluate whether peripheral venous miRNAs represent circulating miRNAs from all blood vessels under a given condition, such as aging, we compared the miRNA profiles of venous and arterial plasma between young and aged rats by Illumina next-generation sequencing. The DEseq2 tool was used to obtain differentially-expressed miRNAs. We observed 105 aging-related deregulated miRNAs in vein and 62 in artery, which were highly associated with cell survival and inflammation, respectively. On the other hand, the young and aged groups exhibited a unique arterial-venous bias. There were 54 differentially-expressed miRNAs in the young group and 42 in the aged group; only 8 miRNAs were shared. Further transcriptional factors enrichment analysis found that the shared miRNAs could be partially upregulated by NF-κB and SIRT1. These transcriptional factors could be organ-specific and/or regulated in physiological and aging states as possible causal factors. This study suggested the potential application of circulating miRNAs, which reflect the systematic response to certain conditions, such as aging, and the importance of origin selection for candidate circulating miRNAs.
循环 miRNA 作为疾病预测和诊断的非侵入性生物标志物受到了广泛关注。然而，大多数样本都来自外周静脉血。为了评估在给定条件（如衰老）下，外周静脉 miRNA 是否代表所有血管中的循环 miRNA，我们通过 Illumina 下一代测序比较了年轻和衰老大鼠静脉和动脉血浆的 miRNA 图谱。使用 DEseq2 工具获得差异表达的 miRNA。我们观察到静脉中有 105 个与衰老相关的下调 miRNA，动脉中有 62 个，分别与细胞存活和炎症高度相关。另一方面，年轻和衰老组表现出独特的动静脉偏倚。年轻组有 54 个差异表达的 miRNA，衰老组有 42 个；只有 8 个 miRNA 是共享的。进一步的转录因子富集分析发现，共享的 miRNA 可以部分由 NF-κB 和 SIRT1 上调。这些转录因子可能是器官特异性的，或者在生理和衰老状态下受到调节，是可能的因果因素。这项研究表明，循环 miRNA 具有潜在的应用价值，它反映了对某些条件（如衰老）的系统反应，以及对候选循环 miRNA 来源选择的重要性。
