Gromkowski S H, Brown T C, Masson D, Tschopp J
Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland.
J Immunol. 1988 Aug 1;141(3):774-8.
It has been shown previously that fragmentation of target cell DNA is an early event in lysis mediated by cytolytic T lymphocytes (CTL). In this study, we have investigated whether CTL-derived granules that exhibit lytic activity also induce DNA fragmentation in murine target cells. Cytolytic granules isolated from three different alloreactive CTL clones were tested for the induction of DNA fragmentation in P815 and EL4 target cells, by using a Triton X-100-facilitated, radiolabeled DNA release assay. In contrast to the CTL clones from which they were derived, the cytolytic granules did not induce DNA fragmentation. Agarose gel electrophoretic analysis of DNA confirmed the lack of discrete DNA fragments in target cells lysed by CTL-derived granules. Possible explanations for the difference in the ability of CTL and CTL-derived granules to trigger DNA fragmentation are discussed.
先前已经表明，靶细胞DNA片段化是细胞毒性T淋巴细胞（CTL）介导的细胞裂解中的早期事件。在本研究中，我们研究了具有裂解活性的CTL衍生颗粒是否也能诱导小鼠靶细胞中的DNA片段化。通过使用Triton X-100促进的放射性标记DNA释放试验，测试了从三种不同的同种异体反应性CTL克隆中分离出的细胞溶解颗粒对P815和EL4靶细胞中DNA片段化的诱导作用。与产生它们的CTL克隆不同，细胞溶解颗粒未诱导DNA片段化。对DNA的琼脂糖凝胶电泳分析证实，在被CTL衍生颗粒裂解的靶细胞中不存在离散的DNA片段。文中讨论了CTL和CTL衍生颗粒触发DNA片段化能力差异的可能解释。
