Center for Protein Studies, Faculty of Biology, University of Havana, Havana, Cuba.
Department of Biological Sciences, University of Calgary, Calgary, Canada.
Z Naturforsch C J Biosci. 2020 Nov 26;75(11-12):397-407. doi: 10.1515/znc-2019-0169.
Metallo-aminopeptidases (mAPs) control many physiological processes. They are classified in different families according to structural similarities. Neutral mAPs catalyze the cleavage of neutral amino acids from the N-terminus of proteins or peptide substrates; they need one or two metallic cofactors in their active site. Information about marine invertebrate's neutral mAPs properties is scarce; available data are mainly derived from genomics and cDNA studies. The goal of this work was to characterize the biochemical properties of the neutral APs activities in eight Cuban marine invertebrate species from the Phyla Mollusca, Porifera, Echinodermata, and Cnidaria. Determination of substrate specificity, optimal pH and effects of inhibitors (1,10-phenanthroline, amastatin, and bestatin) and cobalt on activity led to the identification of distinct neutral AP-like activities, whose biochemical behaviors were similar to those of the M1 and M17 families of mAPs. Additionally, M18-like glutamyl AP activities were detected. Thus, marine invertebrates express biochemical activities likely belonging to various families of metallo-aminopeptidases.
金属氨基肽酶（mAPs）控制着许多生理过程。它们根据结构相似性分为不同的家族。中性 mAPs 催化蛋白质或肽底物 N 末端中性氨基酸的裂解；它们在活性位点需要一个或两个金属辅因子。关于海洋无脊椎动物中性 mAPs 特性的信息很少；可用数据主要来自基因组学和 cDNA 研究。本工作的目的是表征来自门 Mollusca、Porifera、Echinodermata 和 Cnidaria 的八个古巴海洋无脊椎动物物种中性 AP 活性的生化特性。底物特异性、最适 pH 值以及抑制剂（1,10-邻菲啰啉、抑酶肽和最佳抑肽酶）和钴对活性的影响导致了不同中性 AP 样活性的鉴定，其生化行为类似于 mAPs 的 M1 和 M17 家族。此外，还检测到 M18 样谷氨酰 AP 活性。因此，海洋无脊椎动物表达的生化活性可能属于多种金属氨基肽酶家族。
