Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Diseases, Japan.
Influenza Virus Research Center, National Institute of Infectious Diseases, Japan.
Jpn J Infect Dis. 2021 Jan 22;74(1):29-34. doi: 10.7883/yoken.JJID.2020.324. Epub 2020 Jun 30.
The disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in Wuhan, China, in December 2019, has rapidly spread worldwide. SARS-CoV-2 is usually detected via real-time reverse-transcription polymerase chain reaction (RT-PCR). However, the increase in specimen load in institutions/hospitals necessitates a simpler detection system. Here, we present an ultra-rapid, real-time RT-PCR assay for SARS-CoV-2 detection using PCR1100 device. Although PCR1100 tests only one specimen at a time, the amplification period is less than 20 min and the sensitivity and specificity match those of conventional real-time RT-PCR performed on large instruments. The method is potentially helpful when daily multiple SARS-CoV-2 testing is needed, for example to confirm virus-free status prior to patient discharge.
2019 年 12 月，在中国武汉发生的由严重急性呼吸综合征冠状病毒 2 型（SARS-CoV-2）引起的疾病已迅速在全球范围内蔓延。SARS-CoV-2 通常通过实时逆转录聚合酶链反应（RT-PCR）进行检测。然而，医疗机构标本负荷的增加需要更简单的检测系统。在这里，我们使用 PCR1100 设备介绍了一种用于 SARS-CoV-2 检测的超快速实时 RT-PCR 检测方法。虽然 PCR1100 一次只能检测一个标本，但扩增时间不到 20 分钟，其灵敏度和特异性与在大型仪器上进行的常规实时 RT-PCR 相同。当需要每天进行多次 SARS-CoV-2 检测时，例如在患者出院前确认无病毒状态时，该方法可能会有所帮助。
